MBIO 3812 Plaque Assay Flowchart

MBIO 3812 Plaque Assay Flowchart - 3 ml soft agar 3 ml soft...

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9/26/11 Ex 6-5 Plaque Assay of Virus Titer Original phage sample Tube #P1 10-1 Tube #P2 10-2 Tube #P3 10-3 Tube #P4 10-4 Tube #P5 10-5 Tube #P6 10-6 Tube #P7 10-7 Tube #P8 10-8 0.9 ml saline in each tube 0.1 ml 0.1 ml 0.1 ml 0.1 ml 0.1 ml 0.1 ml 0.1 ml 0.1 ml change tip change tip change tip change tip change tip change tip change tip Tube #EP2 Tube #EP3 Tube #EP4 Tube #EP5 Tube #EP6 Tube #EP7 Tube #EP8 0.3 ml E. coli culture in each tube E. coli control Sterile saline ( E. coli + sterile saline) 0.1 ml 0.1 ml 0.1 ml 0.1 ml 0.1 ml 0.1 ml 0.1 ml 0.1 ml Steps 1 and 2:
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9/26/11 Ex 6-5 Plaque Assay of Virus Titer Tube #EP2 Tube #EP3 Tube #EP4 Tube #EP5 Tube #EP6 Tube #EP7 Tube #EP8 E. coli control Step 3: 3 ml soft agar 3 ml soft agar 3 ml soft agar 3 ml soft agar 3 ml soft agar
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Unformatted text preview: 3 ml soft agar 3 ml soft agar 3 ml soft agar entire contents entire contents entire contents entire contents entire contents entire contents entire contents entire contents Each tube at ~45C 9/26/11 Ex 6-5 Plaque Assay of Virus Titer Step 4: entire contents entire contents entire contents entire contents entire contents entire contents entire contents entire contents control from 10-2 tube from 10-3 tube from 10-4 tube from 10-5 tube from 10-6 tube from 10-7 tube from 10-8 tube Once soft agar solidifies turn the plates over and incubate at 37C overnight Plate 2 Plate 3 Plate 4 Plate 5 Plate 6 Plate 7 Plate 8...
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This note was uploaded on 09/26/2011 for the course MBIO 3812 taught by Professor Staff during the Fall '11 term at The University of Oklahoma.

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MBIO 3812 Plaque Assay Flowchart - 3 ml soft agar 3 ml soft...

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