MBIO 3812 Supplement Making Dilutions

MBIO 3812 Supplement Making Dilutions - want to make a 1:10...

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11 MBIO 3812 Making Dilutions 1 : 10 The final volume you want ( i.e ., 10 ml) The amount of sample to add ( i.e. , 1 ml) The amount of solution to add (10 – 1 = 9 ml) What to call a dilution example: 1 in 10 1:10 1/10 10-1 How to make a dilution
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22 Making Dilutions 1 : 10 The final volume you want 10 • 10 = 1 ml The amount of sample to add 1 g 10 = 0.1 ml The amount of solution to add 9 g 10 = 0.9 ml If you want to reduce the volume of the dilution you are making: Divide all the volumes used in the dilution by the same number MBIO 3812 Example:
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Unformatted text preview: want to make a 1:10 dilution but only have 0.1 ml of the sample 33 Making Dilutions Important points to remember: ALWAYS add the sample to the saline or broth ALWAYS mix each dilution tube after adding the sample ALWAYS , ALWAYS CHANGE PIPET TIPS AFTER EACH SAMPLE TRANSFER , not doing this will ruin your dilution series and your experiment! MBIO 3812 44 Making Dilutions change tip change tip change tip change tip change tip saline MBIO 3812...
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MBIO 3812 Supplement Making Dilutions - want to make a 1:10...

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