3813-002Day3Posted - M B IO 3 8 1 3 0 0 2 h o n s Day 3 Fri...

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1 MBIO 3813-002 hons Day 3 Fri 8/26/11; slight (non- substantive) changes to order and content of slides, a few figs removed Topics - What is microbiology What happened up to ca. 1850? What happened from ca. 1850 to 1900? What happened from 1900 to 2000? Life and death expectancy, U.S. For Monday, read about: • Winogradsky Column, Ch 1 Hypotheses and Theories • Cell Theory - Hooke • Bacteria are alive - van Leeuwenhoek • Living cells are formed de novo in contemporary time – (from organic matter, O 2 may be required) • Germs cause Disease • Microorganisms drive ecology Microbiology: An Evolving Science 4 A most elegant experiment 1 1 beauty of form or style; pleasing to sensibility • Louis Pasteur (1822-95) – 1861, took on the spontaneous gen hypot/theory
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2 What is source of cells? • Assay 1: putrefaction of organic solution/solids • Assay 2: microscopic observation of cells in putrefied material • Positive result in 1 or 2: Cells must have come from the organic material (O 2 /air might be implicated, depending on expt’l design) Microbiology: An Evolving Science 6 Disproving/refuting the hypothesis 1861: Cells did not grow in sterile meat broth, even with O
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3813-002Day3Posted - M B IO 3 8 1 3 0 0 2 h o n s Day 3 Fri...

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