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3813-002Day7posted - to p ic s M B IO 3 8 1 3 0 0 2 Day 7...

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1 MBIO 3813-002 Day 7 Wed 9/7/11 Projected Hubbell Space Telescope photo of distant galaxies from Dec 2009 Also Fig 3.1, the bact cell’s components to scale topics Characterizing Microbial Cell Genomics – Microscopy – Biochemistry Separation of components & chem test X-ray & other structural methods – Physiology • measurements Vis light can be: • Reflected • Refracted • Scattered • Absorbed – Chromophore – Fluorophore: λ absorbed < λ emitted λ , visible light, 4 to 7.5 x 10 -7 m Bright-Field Microscopy: Staining Fix cells to hold in position Stain with dye – Reacts with/ binds to a chem structure of cell – Increases absorbance (background is white light) Cells easier to find in low-contrast conditions
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2 Differential Stain Distinguishes among cell types owing to differences in a chemical property Iconic Ex.: Gram Stain Fix 1 0 Stain ( crystal violet ) Iodine (I 2 / I - ) Decolorize, EtOH Counterstain ( saffranin ) A. Acid Fast Stain: Mycobacterium tuberculosis B. Endospore Stain: Bacillus
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