3813-002Day7posted - to p ic s M B IO 3 8 1 3 0 0 2 Day 7 Wed Characterizing Microbial Cell Genomics Projected Hubbell Space Telescope photo of

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
1 MBIO 3813-002 Day 7 Wed 9/7/11 Projected Hubbell Space Telescope photo of distant galaxies from Dec 2009 Also Fig 3.1, the bact cell’s components to scale topics Characterizing Microbial Cell – Genomics – Microscopy – Biochemistry – Physiology • measurements Vis light can be: • Reflected • Refracted • Scattered • Absorbed – Chromophore – Fluorophore: λ absorbed < λ emitted λ , visible light, 4 to 7.5 x 10 -7 m Bright-Field Microscopy: Staining • Fix cells to hold in position • Stain with dye – Reacts with/ binds to a chem structure of cell – Increases absorbance (background is white light) • Cells easier to find in low-contrast conditions
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
2 Differential Stain • Distinguishes among cell types owing to differences in a chemical property • Iconic Ex.: Gram Stain Fix 1 0 Stain ( crystal violet ) Iodine (I 2 / I - ) Decolorize, EtOH Counterstain ( saffranin ) A. Acid Fast Stain: Mycobacterium tuberculosis
Background image of page 2
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 4
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 09/26/2011 for the course MBIO 3813 taught by Professor Staff during the Fall '11 term at The University of Oklahoma.

Page1 / 4

3813-002Day7posted - to p ic s M B IO 3 8 1 3 0 0 2 Day 7 Wed Characterizing Microbial Cell Genomics Projected Hubbell Space Telescope photo of

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online