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Unformatted text preview: • What do you do to study this cell? 2 characterizations • Seq: 16S rRNA gene, or 16S rRNA – More and more, seq some or all of genome – Analyze seq for taxonomic purp • Fractionate cell – Break it apart and separate components by polarity, density, pI, size, chem affinity for separation matrices,… polarity • Solvent extraction, obtain Phospholipids • Chemical hydrolysis of PL OH-, 100° C • What do you get? • Ester bond --> alcohol & acid • Ether bond --> N.R. (try HI and 150° C) Ester (Alkali-labile) The ether links in Archaeal lipids give chemical stability to the membrane (well-needed for cells growing, e.g. at pH 2) The membrane-spanning tetraether stabilizes the membrane at high temps ( e.g. > 80° C). Cell can regulate what % of its lipids are tetraethers as req’d by temperature...
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This note was uploaded on 09/26/2011 for the course MBIO 3813 taught by Professor Staff during the Fall '11 term at The University of Oklahoma.
- Fall '11