This preview shows pages 1–2. Sign up to view the full content.
This preview has intentionally blurred sections. Sign up to view the full version.View Full Document
Unformatted text preview: What do you do to study this cell? 2 characterizations Seq: 16S rRNA gene, or 16S rRNA More and more, seq some or all of genome Analyze seq for taxonomic purp Fractionate cell Break it apart and separate components by polarity, density, pI, size, chem affinity for separation matrices, polarity Solvent extraction, obtain Phospholipids Chemical hydrolysis of PL OH-, 100 C What do you get? Ester bond --> alcohol & acid Ether bond --> N.R. (try HI and 150 C) Ester (Alkali-labile) The ether links in Archaeal lipids give chemical stability to the membrane (well-needed for cells growing, e.g. at pH 2) The membrane-spanning tetraether stabilizes the membrane at high temps ( e.g. > 80 C). Cell can regulate what % of its lipids are tetraethers as reqd by temperature...
View Full Document
- Fall '11