Day12-13Posted - 12 transporter. Fig 3.11, 1st ed Fig 4.3,...

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MBIO 3813-002 Day 13 Wed 9/21/11 Mycobacterium tuberculosis
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Wed 9/21 Poisoner’s Handbook talk today 4 pm 211 Dale Hall Pre-read Ch 4, microbial nutrition Ghost Map topic statement to be released Friday. [Please read the book for enjoyment before biasing your read with the statement!] Exam 1 discussion Fri
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topics Separation & characterization of components Fractionate cell Break it apart and separate components by polarity, density, pI, size, chem affinity for separation matrices,… Micromachines: ATPase, Flagellum Cytoplasm
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density Sucrose gradient centrifugation Ribosomes move in centrifugal field: 30S, 50S subunits, 70S native ribosome in Bact and Archaea 40S, 60S, subunits, 80S native rib in Euk. Inner & Outer Membrane Components separate in density gradient
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Figure 3-23 Outer membrane analysis by centrifugation.
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Figure 3-9 Common drugs are membrane-permeant weak acids and bases.
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Figure 3-10 A membrane-embedded transport protein: the vitamin B
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Unformatted text preview: 12 transporter. Fig 3.11, 1st ed Fig 4.3, 2nd ed See eqn next slide! ATP synth at F1Fo ATP synthase ADP + P i---> ATP + H 2 O AMP~P AMP~P~P pH Gradients pH acidic outside, alkaline inside [H + ] out > [H + ] in e.g. in , pH 7, 1 x 10-7 M ; out , pH 6, 1 x 10-6 M Opposite Case pH alkaline outside, acidic inside e.g. in , pH 7, 1 x 10-7 M ; out , pH 9, 1 x 10-9 M How does this cell harness energy at the membrane? If inside is alkaline relative to outside 1 Localized proton hypothesis (H + from respiration does not equilibrate with bulk water phase outside cell) 2 Use of Na + , sodium ion, in lieu of H + 3 Na + / H + antiporter (when [Na + ] out > [Na + ] out antiporter exchanges Na + for H + ; then may invoke localized proton hypothesis) Biased Random Walk, net result: movement towards attractabt...
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Day12-13Posted - 12 transporter. Fig 3.11, 1st ed Fig 4.3,...

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