Day12-13Printed - M B IO 3 8 1 3 - 0 0 2 W e d 9 /2 1 Day...

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Unformatted text preview: M B IO 3 8 1 3 - 0 0 2 W e d 9 /2 1 Day 13 Wed 9/21/11 • Poisoner’s Handbook talk today 4 pm 211 Dale Hall • Pre-read Ch 4, microbial nutrition • Ghost Map topic statement to be released Friday. [Please read the book for enjoyment before biasing your read with the statement!] • Exam 1 discussion Fri M. tuberculosis to p ic s Separation & characterization of components Fractionate cell – Break it apart and separate components by polarity, density, pI, size, chem affinity for separation matrices,… – Micromachines: ATPase, Flagellum – Cytoplasm d e n s ity • Sucrose gradient centrifugation – Ribosomes move in centrifugal field: 30S, 50S subunits, 70S native ribosome in Bact and Archaea 40S, 60S, subunits, 80S native rib in Euk. • Inner & Outer Membrane Components separate in density gradient 1 EM images of ribosomes Figure 3-23 Outer membrane analysis by centrifugation. 2 Figure 3-9 Common drugs are membrane-permeant weak acids and bases. Fig 3.11, 1st ed Fig 4.3, 2nd ed See eq’n next slide! Figure 3-10, 2 nd ed. A membrane-embedded transport protein: the vitamin B12 transporter. A T P s y n th a t F 1 F o A T P s y n th a s e ADP + Pi ---> ATP + H2O AMP~P AMP~P~P 3 p H G r a d ie n ts • pH acidic outside, alkaline inside [H+]out > [H+]in e.g. in, pH 7, 1 x 10-7 M; out, pH 6, 1 x 10-6 M Opposite Case • pH alkaline outside, acidic inside e.g. in, pH 7, 1 x 10-7 M; out, pH 9, 1 x 10-9 M How does this cell harness energy at the membrane? I f i n s i d e i s a l k a l i n e r e l a t i v e t o o u t s i d e… 1 2 3 Fig 3.44 two views of Bact Flagellae Localized proton hypothesis (H+ from respiration does not equilibrate with bulk water phase outside cell) Use of Na+, sodium ion, in lieu of H+ Na+ / H+ antiporter (when [Na+]out > [Na+]out antiporter exchanges Na+ for H+; then may invoke localized proton hypothesis) 4 Biased Random Walk, net result: movement towards attractabt 5 ...
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Day12-13Printed - M B IO 3 8 1 3 - 0 0 2 W e d 9 /2 1 Day...

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