Chapter 3 - Observing microorganisms through a microscope

Chapter 3 - Observing microorganisms through a microscope -...

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Microscope Chapter 3: Observing Microorganisms Through a Microscope The diameter of most living cells is between 1 and 100 um (micrometer), and therefore are visible only under a microscope. Types of Microscopes 1. Simple Microscope: has only one magnifying lens. 2. Compound Microscope: has more than one magnifying lenses. Functions of Microscope A. Magnification B. Resolution or Resolving Power (Resolving power of a compound light microscope (oil immersion lens) is 0.2 Um. Types of Compound Microscopes 1. Bright Field: it forms a dark image against a bright background. It can be used to observe live or preserved, stained or unstained specimens. 2. Dark Field: Specimen appears light against a dark background. It is commonly used to observe organisms that are sensitive to heat or desiccation. 3. Phase contrast, interference, and confocal: Provides three dimensional image of the specimen. These microscopes are excellent for observing intracellular
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This note was uploaded on 09/24/2011 for the course MCB MCB2010 taught by Professor Smith during the Fall '09 term at Broward College.

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Chapter 3 - Observing microorganisms through a microscope -...

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