Lecture notes for prelim 1 - Lecture2 15:08 Office hours...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 2 15:08 Office hours for lecture material M 10-11:30, wed 12:30-2 106 stimson E-mail- don’t know who to send it to… [email protected] Biology open house Wed Sept 1 st 4-5:30pm Stimson Hall Cells under the microscope Reading: ECB 1-26 and 495-499 Size scales relevant to cell biology Cells- .2mm-2 μ m minimum - resolvable by unaided eye Organelles 2 μ m- 200nm - minimum resolvable by light microscope Molecules 20nm-.2nm Atoms .2nm - minimum resolvable by electron miscroscope Cells can vary enormously in size Streptoccus bacteria ~ 12 μ m diameter Ostrich egg ~ Cells can vary enormously in shape Nerve cell from the cerebellum Paramecium, a giant single-cell organism
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Cells in a plant stem Small bacterium with long flagellum Animal skin cell viwed by light microscopy Section of cells in kidney duct stained with dyes (to see better) Problem with dyes is their specificity is not always known/precise Indirect immunoflourescence Antibodies against protein youre studying so shows up as dye Frog pigment cells stained with fluorescent dyes Used antibody, and then a second antibody Can use different tags to look at several different proteins Disadvantage- have to fix the cells- snapshot in time- not live Green fluorescent protein (GFP) Have a fairly small protein that folds up in a way that becomes fluorescent One protein that has protein A that continues to protein B Liver cell viewed by electron microscopy Take cell/tissue and fix them and slice them really thin get stained with a dye that an electron beam would see Advantage of electron microscopy More detailed
Background image of page 2
Disadvantage to electron microscopy Harder to do Hard to get 3d image More difficult to label Cant use live cells procaryotes single celled can be spherical, spiral shaped, or rod-shaped can live in extreme places as well as non extremes extremely diverse in terms of what they are capable of doing eukaryotes plants, animals, mostly multicellular organisms (also some single-cellular organisms) 2 layers of membrane, interrupted by nuclear pores which allow materials to flow into and out of nucleus membrane-enclosed organelles o mitochondria 2 membranes (high surface area to carry out oxidative phosphorilation) o chloroplast green because they have chlorophyll found in plant cells, in certain algae have 2 membranes o endoplasmic reticulum (ER) think long membrane most lipids and membrane components are made
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
secreted proteins are made in the ER o Golgi apparatus Looks like a stack of pancakes- discs on top of each other Where proteins go once out of ER Modifies proteins by changing them Sorts proteins o Lysosome Break down food o Peroxisomes Break down toxic waste o Cytosol Half the cell once all membrane organelles are removed Mini metabolic processes occur Active part of cell (not only water)
Background image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 09/26/2011 for the course HD 115 at Cornell University (Engineering School).

Page1 / 54

Lecture notes for prelim 1 - Lecture2 15:08 Office hours...

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online