lec16 - Last Time 3/4/08

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Last Time 3/4/08
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Increase (I) concentration increase slope intercept remains unchanged. - r S = V max S ( 29 K M 1 + I (29 K I + S ( 29
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Uncompetitive Inhibition Inhibition only has affinity for enzyme-substrate complex Developing the rate law (1) (2)   E + S k 1  → k 2 ←  E S k cat  →  P I + E S k 4  → k 5 ←  I E S r P =- r S = k ca t E S ( 29 r E S = 0 = k 1 E ( 29 S ( 29 - k 2 E S (29 - k ca t E S (29 - k 4 I ( 29 E S (29 + k 5 I E S ( 29 r I E S = 0 = + k 4 I ( 29 E S ( 29 - k 5 I E S ( 29
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Adding (1) and (2) k 1 E ( 29 S (29 - k 2 E S ( 29 - k cat E S ( 29 = 0 E S ( 29 = k 1 E ( 29 S (29 k 2 + k cat = E ( 29 S (29 K M I E S ( 29 = k 4 k 5 I (29 E S ( 29 = I (29 E S ( 29 K I = I (29 E ( 29 S ( 29 K I K M K I = k 5 k 4 r p = k cat E S ( 29 = k cat E ( 29 S ( 29 K M From (2)
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Total enzyme E t = E ( 29 + E
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This note was uploaded on 09/28/2011 for the course CHEMICAL E 534 taught by Professor Fogler during the Spring '11 term at University of Michigan-Dearborn.

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lec16 - Last Time 3/4/08

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