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Unformatted text preview: Problem Set 8 Ch. 9 1. Five Hfr strains of E. coli (A to E) derived from the same F + strain are mated with a female strain. The table below shows the times of entry of the first 5 genes in an interrupted mating experiment. Hfr Strains . A B C D E . Gene Time Gene Time Gene Time Gene Time Gene Time mal 1 ade 13 pro 3 pro 10 his 7 str s 11 his 28 met 29 gal 16 gal 17 ser 16 gal 38 xyl 32 his 26 pro 23 ade 36 pro 44 mal 37 ade 41 met 49 his 51 met 70 str s 47 ser 61 xyl 52 . a. Draw a map of the male strain of E. coli, indicating the positions of all genes and the distances between them in minutes. b. Show the insertion point and orientation of the F-factor (plasmid) for each Hfr strain. 2. An Hfr strain of genotype a + b + c + d- str s was mated with a F- strain of the genotype a- b- c- d + str r (a- mutant cannot synthesize nutrient A, b- mutant cannot synthesize nutrient B, etc.)....
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This note was uploaded on 09/29/2011 for the course GENETICS 380 taught by Professor Glodowski during the Spring '08 term at Rutgers.
- Spring '08