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Problem Set 8 Answers - Genetics 380 Spring 2011 Problem...

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1. Maps derived from interrupted mapping data- 1 10 5 20 15 Strain A- <-|----------|-----|--------------------|---------------|-------- mal str s ser ade his 13 15 10 6 26 Strain B <-------------|---------------|----------|------|--------------------------|--- ade his gal pro met 3 26 3 5 10 Strain C <---|--------------------------|---|-----|----------|----------- pro met xyl mal str s 20 15 10 6 10 Strain D ---------|--------------------|----------------|----------|------|----------> ser ade his gal pro 7 10 6 26 3 Strain E <-------|---------|------|--------------------------|---|------ his gal pro met xyl The combined map is shown below- Genetics 380 : Spring 2011 Problem Set 8 Answers
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2. (a) Gene c + transferred first (starting at 5 minutes), gene b + second (starting at 10 minutes) and gene a + is transferred last (starting at 20 minutes). ______ a +_____ b +___ c + _____ > (b) a + d + colonies = 51/100 b + d + colonies = 8/100 c + d + colonies = 89/100 The parental combination of genes was a + b + c + d - (donor) and a - b - c - d + (recipient). The data above say that there is 8% recombination between genes d and b; 51% recombination between genes d and a ; and 89% recombination between genes d and c . Therefore, gene 'd' is closest to gene 'b' and closer to gene 'a' than gene 'c' . The map derived from these data is- Even though the above is the likely map. The data don’t rule out the alternative map- _____a + _________b + __d - ___________c + _____ > 8 89 --------51------- 3.
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