{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Problem Set 12 Answers

Problem Set 12 Answers - Genetics 380 Spring 2011 Problem...

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Problem Set 12 Answers 1. (a) Number of cuts in linear DNA is 1 less than the number of pieces observed. Therefore, there are 2 cuts each made by BamHI and HindIII in this linear DNA. (b) In the restriction map below, B indicates BamHI cuts and H indicates HindIII cuts. Also, the size of each piece is shown in kb. 1.5 | 1.8 | 1.2 . 1.5 H 1.0 0.8 H 0.5 0.7 |------------------------------|--------------------|------------------|------------|--------------| B B 2.5 | 1.3 | 0.7 . 2. (a) For a circular DNA (such as a plasmid), the number of pieces is equal to the number of cuts made by a restriction nuclease. Therefore, EcoRI and BamHI make one cut each and SalI makes 3 cuts. (b) The map is shown below. The sizes for the DNA pieces are in kb and the 3 restriction enzymes are shown by one letter abbreviations (B = BamHI; E = EcoRI; S = SalI). | E | B S S S 2.2 2.5 1.4 0.7 1.0 Genetics 380 : Spring 2011
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
(b) The tet R gene used as a probe will detect only those bands that contain tet R DNA. Thus, all bands in the control lanes will have sequences complementary to the probe. For the clone15 digests, the HindIII 5-kb band will be radioactive and both EcoRV bands will be radioactive. For the HindIII + EcoRV double digest, the 3-kb and 2-kb bands will be radioactive.
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern