Problem Set 12 Answers

Problem Set 12 Answers - Genetics 380 : Spring 2011 Problem...

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Problem Set 12 Answers 1. (a) Number of cuts in linear DNA is 1 less than the number of pieces observed. Therefore, there are 2 cuts each made by BamHI and HindIII in this linear DNA. (b) In the restriction map below, B indicates BamHI cuts and H indicates HindIII cuts. Also, the size of each piece is shown in kb. 1.5 | 1.8 | 1.2 . 1.5 H 1.0 0.8 H 0.5 0.7 |------------------------------|--------------------|------------------|------------|--------------| B B 2.5 | 1.3 | 0.7 . 2. (a) For a circular DNA (such as a plasmid), the number of pieces is equal to the number of cuts made by a restriction nuclease. Therefore, EcoRI and BamHI make one cut each and SalI makes 3 cuts. (b) The map is shown below. The sizes for the DNA pieces are in kb and the 3 restriction enzymes are shown by one letter abbreviations (B = BamHI; E = EcoRI; S = SalI). | E | B S S S 2.2 2.5 1.4 0.7 1.0 Genetics 380 : Spring 2011
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(b) The tet R gene used as a probe will detect only those bands that contain tet R DNA. Thus, all bands in the control lanes will have sequences complementary to the probe. For the clone15 digests, the
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Problem Set 12 Answers - Genetics 380 : Spring 2011 Problem...

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