Exam 1A_2010 - Genetics 380, Spring 2010 Examination #1 A...

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Genetics 380, Spring 2010 Name_________________________ Examination #1 A Student #______________________ Feb. 16, 2010 Section _______________________ Q. 1 . (a; 6 points) List the 3 main differences between DNA and RNA? ( b; 4 points) Draw the structure of Guanine. Be sure to label the positions in the ring structure. Q. 2. (10 points) Given DNA with 25% each of A, C, G and T, how far apart will the 2 restriction enzymes cut? Human DNA is 40% GC and 60% AT. How far apart will the cuts be now? (R = Purine; Y = Pyrimidine) Average DNA Human DNA (a) HaeII RGCGCY ______________ _____________ (b) EcoRI GAATTC ______________ _____________
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Q. 3 . ( 10 points) DNA was analyzed from a large number of humans by digestion with EcoR I enzyme, Southern blotting and probing with a cloned radioactive DNA probe. Ten different restriction fragment distribution patterns (polymorphisms) were observed as shown in the gel below. How many different alleles of this locus are present in the population (use symbols like A1, A2, A3, etc. to denote alleles)? Assign a genotype to each of the 10 individuals shown in the gel below. Remember that humans are diploid and any one person can have only 2 alleles of a gene. # of alleles in the population ______ Lane # Genotype 1 ________ 2 ________ 3 ________ 4 ________ 5 ________ 6 ________ 7 ________ 8 ________ 9 ________ 10 ________
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Q. 4 ( 6 points ) In Peas, Purple flower (P) is dominant over white (p). Round seeds (W) are
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This note was uploaded on 09/29/2011 for the course GENETICS 380 taught by Professor Glodowski during the Spring '08 term at Rutgers.

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Exam 1A_2010 - Genetics 380, Spring 2010 Examination #1 A...

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