Exam 1A_Answer_2010

Exam 1A_Answer_2010 - Genetics 380, Spring 2010 Examination...

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Genetics 380, Spring 2010 Answer Key Examination #1 A Q. 1 . (a; 6 points) List the 3 main differences between DNA and RNA? ( b; 4 points) Draw the structure of Guanine . Be sure to label the positions in the ring structure. (a) (i) Sugar- deoxyribose in DNA; ribose in RNA (ii) Base- T in DNA; U in RNA (iii) DNA- usually Double-stranded; RNA- usually single-stranded It is not necessary to show all the H or the double bonds. Two rings (6-member and 5-member) should be shown (with the right number of C and N) and the 2-amino and 6-keto should be shown for guanine. Ring positions were only numbered for Adenine in the book. So, I am also attaching Adenine here. Q. 2. (10 points) Given DNA with 25% each of A, C, G and T, how far apart will the 2 restriction enzymes cut? Human DNA is 40% GC and 60% AT. How far apart will the cuts be now? (R = Purine; Y = Pyrimidine) Average DNA Human DNA (a) HaeII RGCGCY ____1,024___ ____2,500 ____ (b) EcoRI GAATTC ____4,096____ ____3,086 ____ Hae II 1/[(0.5) 2 (0.25) 4 ] 1/[(0.5) 2 (0.2) 4 ] EcoRI 1/(0.25) 6 1/[(0.2) 2 (0.3) 4 ]
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Q. 3 . ( 10 points) DNA was analyzed from a large number of humans by digestion with EcoR I enzyme, Southern blotting and probing with a cloned radioactive DNA probe. Ten different restriction fragment distribution patterns (polymorphisms) were observed as shown in the gel below. How many different alleles of this locus are present in the population (use symbols like A1, A2, A3, etc. to denote alleles)? Assign a genotype to each of the 10 individuals shown in the gel below. Remember that humans are diploid and any one person can have only 2 alleles of a gene. # of alleles in the population __ 4_ Lane # Genotype 1 ____A1 A1_____ 2 ____A2 A2_____ 3 ____A3 A3_____ 4 ____A4 A4____ 5 ____A1 A2____ 6 ____A1 A3____ 7 ____A1 A4____ 8 ____A2 A3____ 9 ____A2 A4____ 10 ____A3 A4____ Hybridization |___Probe____| Not required of Students (for explanation only)- A1 |_____3.1____ _ __|__1.9____| A2 |__________5____________| A3 | 1 | 4 _ | A4 | 1 | 2.1 - | 1.9 |
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Q. 4 ( 6 points ) In Peas, Purple flower (P) is dominant over white (p). Round seeds (W) are
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This note was uploaded on 09/29/2011 for the course GENETICS 380 taught by Professor Glodowski during the Spring '08 term at Rutgers.

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Exam 1A_Answer_2010 - Genetics 380, Spring 2010 Examination...

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