Exam 3A_2010 Answers

Exam 3A_2010 Answers - Exam #3A ANSWER KEY Genetics 380...

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Genetics 380 :Spring 2010 Exam #3A ANSWER KEY May 11, 2010 1 . ( 15 points ) . Genetic Constitution Z activity Y activity . -lac +lac -lac +lac . i + o + z + y + 1 100 1 100 i - o + z + y + _100_ _100 __ __100 _ _100 _ i + o c z + y + _100_ _100 __ __100 _ _100 _ i s o + z + y + _1_ _1 __ __1 _ _1 _ i + o + z + y - _1_ _100 __ __1 _ _1 _ i + o + z - polar y + _1__ __1 __ ___1 _ ___1 __ i s o c z + y + _100_ _100 __ __100 _ _100 _ i - o + z + y - / i + o + z - y + _1_ _100 __ __1 _ _100 _ i + o + z + y - /i + o c z - y + _1_ _100 __ __100 _ _100 _ i s o + z + y - /i + o + z - y + _1_ _1 __ __1 _ _1 _ i s o + z + y - / i + o c z - y + _1_ _1 __ 100 _ _100 _ 2. (9 points) The questions below are about the tryptophan operon of E. col i . (a) Is it an inducible or a repressible operon? ______ repressible ______ (b) Is it a positively or negatively regulated? ______ negatively _________ (c) Does this operon have a second mechnism of regulation? If yes, name the mechanism. _______ attenuation _________ (d) What is the corepressor in the trp operon? tryptophan (+ trp-tRNA) (e) Which regions in the leader sequence have to pair in order to make a long mRNA? ___ ____ (f) Which regions in the leader sequence have to pair in order to make a short mRNA? ___ ____
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3. (12 points) A circular plasmid gives the DNA bands of the sizes shown in the table below, when digested with 3 restriction enzymes (either singly or in various combinations). Draw a restriction map of the circular plasmid. Enzyme(s) DNA band size (kb) EcoRI (E) 12 SalI (S) 8, 4 ClaI (C) 9, 3 SalI + EcoRI (S+E) 8, 3, 1 SalI + ClaI (S+C) 4, 4, 3, 1 EcoRI + ClaI (E+C) 7, 3, 2 EcoRI + ClaI + SalI (E+C+S) 4, 3, 3, 1, 1
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4. (12 points) Shown below is a human pedigree in which an inherited genetic disease is segregating. DNA samples were prepared from each of the persons in the pedigree. These DNA samples were digested with a restriction enzyme, the fragments separated in an agarose gel and a blot was prepared from the gel. The blot was probed with a radioactive probe using a cloned DNA sequence called RS1234. The results of the Southern blot are shown below the pedigree. Based on these results, answer the questions below- (a) ( 2 pt) What is the mode of inheritance of this disease? Autosomal dominant, Autosomal recessive, X-linked dominant, X-linked recessive, Y-linked dominant or Y-linked recessive. Autosomal dominant
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This note was uploaded on 09/29/2011 for the course GENETICS 380 taught by Professor Glodowski during the Spring '08 term at Rutgers.

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Exam 3A_2010 Answers - Exam #3A ANSWER KEY Genetics 380...

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