Exam 3A_2010 - Genetics 380 :Spring 2010 Name (print) _...

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Genetics 380 :Spring 2010 Name (print) __________________________ Exam #3A Rutgers ID # _________________________ May 11, 2010 Section (TA) __________________ 1 . ( 15 points ) . Genetic Constitution Z activity Y activity . -lac +lac -lac +lac . i + o + z + y + 1 100 1 100 i - o + z + y + _____ _____ _____ _____ i + o c z + y + _____ _____ _____ _____ i s o + z + y + _____ _____ _____ _____ i + o + z + y - _____ _____ _____ _____ i + o + z - polar y + _____ _____ _____ _____ i s o c z + y + _____ _____ _____ _____ i - o + z + y - / i + o + z - y + _____ _____ _____ _____ i + o + z + y - /i + o c z - y + _____ _____ _____ _____ i s o + z + y - /i + o + z - y + _____ _____ _____ _____ i s o + z + y - / i + o c z - y + _____ _____ _____ _____ 2. (9 points) The questions below are about the tryptophan operon of E. col i . (a) Is it an inducible or a repressible operon? ________________________ (b) Is it a positively or negatively regulated operon? ________________________ (c) Does this operon have a second mechnism of regulation? If yes, name the mechanism. ________________________ (d) What is the corepressor in the trp operon? ________________________ (e) Which regions in the leader sequence (1,2,3,4) have to pair in order to make a long mRNA? ___ __ ____ (f) Which regions in the leader sequence have to pair in order to make a short mRNA? _________
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3. (12 points) A circular plasmid gives the DNA bands of the sizes shown in the table below, when digested with 3 restriction enzymes (either singly or in various combinations). Draw a restriction map of the circular plasmid. Caution : A single size band on the gel may contain multiple fragments of the same size. Enzyme(s) DNA band size (kb) EcoRI (E) 12 SalI (S) 8, 4 ClaI (C) 9, 3 SalI + EcoRI (S+E) 8, 3, 1 SalI + ClaI (S+C) 4, 4, 3, 1 EcoRI + ClaI (E+C) 7, 3, 2 EcoRI + ClaI + SalI (E+C+S) 4, 3, 3, 1, 1
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4. (12 points) Shown below is a human pedigree in which an inherited genetic disease is segregating. DNA samples were prepared from each of the persons in the pedigree. These DNA samples were
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Exam 3A_2010 - Genetics 380 :Spring 2010 Name (print) _...

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