Lecture III - Lecture III 1. Types of DNA markers present...

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Lecture III 1. Types of DNA markers present in genomic DNA a. Single nucleotide polymorphism (SNP) b. Restriction fragment length polymorphism (RFLP) c. Tandem repeat polymorphisms i. Simple sequence repeat (SSR) ii. Variable number of tandem repeats 2. RFLPs are due to SNPs in restriction sites a. Mutation at the cleavage point = no cleavage so longer fragment i. Size of piece on gel is different (analyzed in gel) 3. RFLPs are codominant a. Create different sized fragments b. Fragments same size in homozygotes c. Fragments different size in heterozygotes 4. Tandem repeat polymorphism a. Large repeats is shown by a large band (bottom) b. Little repeats is shown by a small band (top) c. Microsatellite DNA (same as simple sequence repeats) d. Repeated DNA i. (1-9)nt repeats = simple repeats e. (ATATATATATAT) n or (CCGCCGCCG) n f. VNTR = variable number of tandem repeats i. >10nt and <100nt = minisatellite DNA 5. Disease genes can be mapped using DNA markers Chapter 3: Transmission Genetics – The Principle of Segregation 1. The 7 traits in garden pea studied by Mendel
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This note was uploaded on 09/29/2011 for the course GENETICS 380 taught by Professor Glodowski during the Spring '08 term at Rutgers.

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Lecture III - Lecture III 1. Types of DNA markers present...

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