RelativeResourceManager5 - RESTRICTION MAPPING Let us start...

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Unformatted text preview: RESTRICTION MAPPING Let us start simply: Example 1. The linear DNA fragment shown here has cleavage sites for Bam HI and Eco RI. In the accompanying diagram of an electrophoresis gel, indicate the positions at which bands would be found after digestion with: a. Bam HI alone b. Eco RI alone c. Bam HI and Eco RI together The dashed lines on the right indicate the positions which bands of 1 - 12 kb would migrate. Now try another one. Example 2. The circular DNA molecule shown below has cleavage sites for Bam HI and Eco RI. In the accompanying diagram of an electrophoresis gel, indicate the positions at which bands would be found after digestion with: a. Bam HI alone b. Eco RI alone c. Bam HI and Eco RI together. The dashed lines on the right indicate the positions which bands of 1 - 12 kb would migrate. First, look at the gel, and determine the size fragments that are present in each lane. I have put them in the following table Bam HI 7 kb, 3 kb Eco RI 7 kb, 3 kb Eco RI + Bam HI 3 kb, 2 kb Now, use the following steps as a guide to help you Try this one:...
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This note was uploaded on 09/28/2011 for the course CHEM 4241 taught by Professor Kreller during the Spring '11 term at Georgia Southern University .

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RelativeResourceManager5 - RESTRICTION MAPPING Let us start...

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