My_Ch20_lecture(2009030423522635)

My_Ch20_lecture(2009030423522635) - Chapter 20: DNA...

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Unformatted text preview: Chapter 20: DNA Technology Purpose : to understand the different types of DNA technology, the function of each technique and how it is used to give scientists detailed genetic information What you MUST know in Ch 20: DNA cloning (overview) Restriction enzymes Vector Probe cDNA Gel electrophoresis RFLPs DNA sequencing Gene therapy DNA fingerprint (= DNA profiling) You will be held responsible for the entire chapter, including PCR—a method of artificially amplifying (making multiple copies of) DNA. For the AP test, you should concentrate your review on the above topics. DNA Cloning • Cloning is used to make multiple identical copies of a gene to: o Make a particular protein Medical use, such as making insulin Academic research use o Make multiple copies of the gene itself To give an organism a new metabolic capability • For example, to confer resistance to a plant disease to a particular plant for agriculture Academic research use, gene sequencing • Restriction enzymes are used to make recombinant DNA o cut DNA at specific locations (the enzyme recognizes a specific recognition site or sequence on DNA) o cuts are made in a staggered way, creating sticky ends o each specific restriction enzyme creates the same set of restriction fragments when DNA is exposed to that enzyme o DNA ligase then splices the new DNA into the host DNA (see fig 20.2, p 366) Cloning Procedure See fig 20.3, p 367 http://www.accessexcellence.org/RC/VL/GG/plasmid.html cloning vector = bacterial plasmid DNA that can carry foreign DNA into a host cell and replicate there Cloning steps: 1.Isolate the bacterial plasmid cloning vector 2.Isolate the human gene that you want to clone2....
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This note was uploaded on 09/29/2011 for the course BIO 020.152 taught by Professor Pearlman during the Fall '08 term at Johns Hopkins.

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My_Ch20_lecture(2009030423522635) - Chapter 20: DNA...

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