greatplatecount

greatplatecount - Yanting Wang 2010 BIOMI 3910 September...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Yanting Wang September 29, 2010 BIOMI 3910 Great Plate Count Anomaly Introduction: For any given environment, only a small portion of bacteria can be cultured using standard plating conditions. The “Great Plate Count Anomaly” study serves to explore the various factors that influence the ability to recover bacteria from environmental samples. Two types of methods for quantifying recovery, CFU and direct count through DAPI staining, were compared. Material and Methods: Study Site: Samples were collected from Houston Pond, a rain-fed freshwater pond located on Cornell University campus. The principle site has been described to contain various vegetations such as water lilies, pickerelweed, yellow flag iris, and bald cypress, as well as a variety of life forms such as reptiles, amphibians, insects, fish, and crustacean (Cornell Plantations, 2010). During sampling, the pH of the water at various locations was found to be approximately 8. Pond surface water temperature measured at 24°C with a dissolved oxygen (DO) content of .8ml/L (oxygen saturation of 8.5 mg/L). Water at the bottom of the pond measured 18°C with a dissolved oxygen content of .7mg/L (oxygen saturation at 9.5mg/L). Sample Collection: Water samples were collected near the surface of the pond in 50-mL sterile centrifuge tubes on August 30, 2010. The sampling location contained an abundance of cattail weed. Samples were stored at room temperature. Growth: Two undefined, complex agar media (Nutrient Agar and Plate Count Agar), two treatments (5-minute sonication and no sonication), and two dilutions (1:1 and 1:9) were used as shown in figure 1 below. Figure 1: Experimental Scheme
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 09/30/2011 for the course BIOMI 3910 at Cornell.

Page1 / 4

greatplatecount - Yanting Wang 2010 BIOMI 3910 September...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online