{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

BCH361_F08 - Translation in Prokaryotes Translation of mRNA...

Info icon This preview shows pages 1–17. Sign up to view the full content.

View Full Document Right Arrow Icon
Translation in Prokaryotes
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Translation of mRNA into the protein sequence 5’ 3’ Ribosome m-RNA growing polypeptide chain NH 2 aminoacyl-tRNA- synthethase tRNA aminoacid aminoacyl-tRNA
Image of page 2
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Activation of amino acids
Image of page 4
Loading of the tRNAs with amino acids by the Aminoacyl-tRNA synthase
Image of page 5

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Step1: a phosphodiester bond is formed between the carboxyl group of the amino acid and the phosphate group of AMP E + ATP + AA PPi + E: AA-AMP E = Enzyme AA = amino acid
Image of page 6
Class II enyzmes: direct formation of the final loaded aminoacyl-tRNA in one further step
Image of page 7

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Class II enzymes Step 2: nucleophilic attack of the free electron pair at 3’OH of last nucleotide of tRNA (anedine) to the carboxyl-C of the amino acid Release of AMP
Image of page 8
Class II enzymes Leading to: Loaded aminoacyl-tRNA
Image of page 9

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Class I enyzmes [aminoacyl-tRNA formation includes 2 steps (step 2 and 3)]
Image of page 10
Class I enzymes Step 2: nucleophilic attack of the free electron pair at 2’OH of last nucleotide of tRNA to the Carboxyl C of the amino acid Release of AMP
Image of page 11

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Class I enzymes Leading to: !!! Amino acid bound to “wrong” position (2’ instead 3’) of the ribose!!!
Image of page 12
Transesterification Class I enzymes Step 3: transfer of the ester bond between the amino acid and the tRNA from 2’ to 3’ position
Image of page 13

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Anticodon loop Recognition sites for the aminoacyl- tRNA synthases
Image of page 14
Extra-Arm (not present in all tRNAs) Two or more DHU (Dihydrouracil) at different positions T ψ C-Arm contains T ψ C sequence aminoacid-Arm Anticodon-Arm
Image of page 15

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Structure of the Gln-tRNA-synthase tRNA Gln as well as ATP are bound to the enzyme The enzyme forms strong interactions with the the anticodon loop and the aminoacyl end of the t-RNA
Image of page 16
Image of page 17
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern