F08-BCH361-L6

F08-BCH361-L6 - Lecture 6 |Enzyme Kinetics II and Enzyme...

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Lecture 6 |Enzyme Kinetics II and Enzyme regulation
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V max [S] V = —————— K M + [S] Michaelis-Menten Equation [E] [S] ([Et] - [ES]) [S] K M =  =  [ES] [ES]
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[E] [S] ([Et] - [ES]) [S] K M =  =  [ES] [ES] [E] >> [ES]
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[E] [S] ([Et] - [ES]) [S] K M =  =  [ES] [ES] [E] = [ES]
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[E] [S] ([Et] - [ES]) [S] K M =  =  [ES] [ES] [E]-> 0 [Et] = [ES]
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Linearizing the Michaelis-Menten Equation
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V max [S] V = —————— K M + [S] 1 K M + [S] ——— = —————— V V max [S] form the reciprocal 1 K M [S] K M 1 1 —— = ———— + ———— = —— —— + —— V V max [S] V max [S] V max [S] V max
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1 K M 1 1 —— = —— —— + —— V V max [S] V max Lineweaver-Burk form of the Michaelis Menten equation the equation has the form of a straight line y = m x + b
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y = m x + b 1 K M 1 1 —— = —— —— + —— V V max [S] V max y = m x + b y x
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Enzyme Inhibition
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This note was uploaded on 09/30/2011 for the course BCH 361 taught by Professor Fromme during the Fall '08 term at ASU.

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F08-BCH361-L6 - Lecture 6 |Enzyme Kinetics II and Enzyme...

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