F08-BCH361-L9

F08-BCH361-L9 - transcription eye (bubble) The rho protein...

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Transcription in Procaryotes
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DNA => RNA => Protein Transcription Translation
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Schematic overview Eye of transcription Non-coding strand Coding strand RNA-DNA Hybrid Un-winding Re-winding
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====== > PPi + mechanism of polymerase reaction Nucleophilic attack
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Prokaryotes The sequence is shown for the coding strand.
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Some examples for promoter regions
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Initiation of Transcription
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1.STEP : Chain Initiation The RNA Holoenzyme screens the DNA for the initiation regions, the σ factor recognizes the initiation region
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2. STEP: Unwinding She unwinds a short piece of DNA
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Elongation of Transcription
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3. Step: Chain elongation Binding of Nucleotidtriphosphates Formation of the phosphodiesterbond, sigma factor is released after formation of 4 base pairs prolongation along the coding strand
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Termination of Transcription
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4. STEP: Termination Termination signals are recognized and the transcription is terminated, polymerase dissociates from DNA
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Termination of the Transcription in Procaryotes (A)By inverted repeats in the sequence
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Termination of the Transcription in Procaryotes ( B) By the rho protein
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Topoisomerase removes supercoils that would form ahead and behind the
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Unformatted text preview: transcription eye (bubble) The rho protein has three domains,two of the bind RNA Inhibition of Transcription Actionomycin D inhibits procaryotic and eucaryotic transcription Regulation of Transcription in Procaryotes Structural genes Repressor protein binds to the operator, Promoter is blocked, gene is inactive Inducer changes the conformation of the repressor protein, it dissociates from the operator, gene is activated One example of positive and negative control: The lactose operon RNA polymerase can only bind in the presence of the cap protein and c-AMP Structure of the lac-repressor and the Cap protein, bound to DNA CAP DNA Repressor post-transcriptional modification of the t-RNA transcripts Many t-RNAs are co-transcribed as larger precursors and have to be spliced to yield the final individual t-RNAs Many t-RNAs are co-transcribed as larger precursors and have to be spliced to yield the final individual t-RNAs Many t-RNAs are co-transcribed as larger precursors and have to be spliced to yield the final individual t-RNAs...
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This note was uploaded on 09/30/2011 for the course BCH 361 taught by Professor Fromme during the Fall '08 term at ASU.

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F08-BCH361-L9 - transcription eye (bubble) The rho protein...

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