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F08-BCH361-L10

F08-BCH361-L10 - Transcription in Eucaryotes DNA Nucleus...

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Transcription in Eucaryotes
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DNA Nucleus Processing hn-RNA Cytosol Ribosomes Protein
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Architecture of yeast polymerase II
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Promotor region of eucaryotes
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TBP TAF A B F E H T ranscription Initiation F actors of RNA Polymerase II TFII T ata- B ox-Binding- P rotein TBP T ata-Box-Binding-Protein- A ssociated- F actor TAF IIS
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TATA Box binding protein TBP
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1. Step: TFII-D, containing the TATA binding protein TBP, binds to the TATA Box
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Step 2: Transcription factors A and B bind
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Step 3: RNA-polymerase II binds to the complex as well as Factor F
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Step 4: Factors E and H bind to the pre- initiation complex
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Step 5: DNA strands separate the C-terminal domain of the polymerase is phosphorylated formation of an open complex
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Overview 90°
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Pol II-DNA/RNA interactions Q conserved in Pol I, II and III Q invariant in Pol I, II and III m-RNA coding DNA strand non-coding DNA strand
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NTP selection difference electron density map after incubation with NTP analogue m-RNA coding DNA strand non-coding DNA strand
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NTP selection (mismatched NTP) coding base mismatched NTP (Westover et al., 2004)
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NTP selection (matched NTP) coding base matched NTP in preinsertion site (Kettenberger et al., 2004)
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NTP selection (matched nucleotide) coding base matched NTP ready for incorporation (Westover et al., 2004) pyrophosphate
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Overview 90°
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