F08-homework_7

F08-homework_7 - (7) Describe the experimental steps for a...

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Homework 7 Fall 2008 BCH 361 Name of the student: (1) What is the main defect of the DNA caused by UV radiation? (2) Describe the different steps in base excision repair. (3) Name three cytotoxic mutations (4a) Explain the steps involved in Transcription Coupled Nucleotide Excision Repair (TC-NER) (4b) Name one disease which is related to defects in the TC-NER. Does the defect leads to premature aging or high cancer disposition? (5) What is the function of a restriction endonuclease in gene technology?
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(6) What protects endogenous DNA from cleavage by its own endonucleases?
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Unformatted text preview: (7) Describe the experimental steps for a cloning vector! (8) Describe the elements of a cloning vector and an expression vector (9) Name two aims of genetic engineering! (10) Name two problems which have to be solved before human proteins can be produced by bacteria! (11) What is the difference between a DNA and a cDNA library? (12) What is the difference between ex vivo and in vitro gene therapy? (13) Describe the major experimental steps which are used for growth of transgenic mice!...
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This note was uploaded on 09/30/2011 for the course BCH 361 taught by Professor Fromme during the Fall '08 term at ASU.

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F08-homework_7 - (7) Describe the experimental steps for a...

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