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Microbial Enumeration Presentation

Microbial Enumeration Presentation - Microbial Enumeration...

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Microbial Enumeration Harrison FDST 4030/6030 1 Microbial Enumeration FDST 4030/6030 Microbial Enumeration What is the significance of trying to enumerate microbes in foods What type of food What type of microbial group Spoilage microorganisms Indicator microorganisms Pathogenic microorganisms Desirable microorganisms (e.g., desired fermentative ones) Microbial Enumeration Translating the ‘counts’ to meaningful conclusions Are there legal requirements Are there ‘customer’ specifications Question – You’re in charge of purchasing raw ingredients that will be used in a finished product that your company processes. What specs would you suggest? Microbial Enumeration Method selection Selective and differential desired, not always applicable Costs Time required to obtain results Reliability of the method Reliability and consistency of supplies needed for the method Training – Complexity – Ease-of-use Microbial Enumeration Direct Microscopic Count (DMC) Most Probable Number (MPN) Membrane Filtration Plating Microbial Enumeration - Direct Microscopic Count (DMC) Advantages Rapid Simple after training Disadvantages Cannot distinguish between live and dead cells
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