Bio Lab report 1

Bio Lab report 1 - I nvestigating enzyme properties in h...

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Investigating enzyme properties in hydrolysis and oxidation-reduction chemical reactions Chris Fox 002:010:A06 9/8/2011
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Abstract Background : When enzymes deal with chemical reactions numerous properties must be taken into consideration: enzyme specificity, enzyme-substrate complex, and that enzymes are dependent on concentration, temperature, and hydrogen ion concentration. Hypotheses 1: Tube a will show the most abundant free amino groups of the three test tubes on the TLC paper. 2: Because of competitive inhibition test tube three will not be colorless. 3: MB (blue) will still be present in tube four at the end of the experiment. 4: Tube two will produce MB-H2 through the enzymatic activity. Secondary purpose 5: Identifying unknown A on the TLC plate. Methods 1: A chromatogram was used to identify free amino groups. 2: Various solutions were mixed to show different enzyme properties. Secondary purpose 3 : Calculated Rf values of amino acid standards from the TLC plate to determine the unknown. Results 1: Hypothesis supported; tube a showed most free amino groups after stain was sprain on TLC plate. 2: Hypothesis supported; Malonic and Sucinnic acid competed for enzyme.
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3: Hypothesis supported; the boiled meat prevented the succinic dehydrogenase from acting upon Sucinnic acid. 4: Hypothesis not supported; tube two did not become colorless after incubation. Secondary purpose 5: Unknown similar to that of tube a and leucine. Conclusions: 1: Tube a produced best results due to enzymatic catalyst. 2: Competitive inhibition prevented enzymes from catalyzing the chemical reaction. 3: The boiled meat was an inhibitor in the chemical reaction. 4: Tube 2 did reduce some MB however the final solution was not colorless. Secondary purpose 5: unknown A is leucine. Introduction An enzyme functions as a catalyst in a chemical reaction. Catalysts accelerates reactions without being consumed in the process, various proteins can function as an enzyme. Without enzymes many chemical reactions in cells would occur to slow to sustain life (4). If a chemical reaction is reversible, the enzyme accelerating the reactants to products may also accelerate the reverse reaction as well (1). Because of this reversibility, only the speed of the reaction is influenced and not the final equilibrium. In this lab we performed a hydrolysis and an oxidation-reduction reaction. In the hydrolysis reaction the enzyme attaches to the substance breaking the substrate down into amino acids. A substrate is the element or molecule the enzyme stimulates in the chemical reaction. Living systems actually control their activity through enzymes (2). Once the all the substrates were broken
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down, the amino acids were identified by chromatography. Chromatography is the process of molecules being separated from the solution based on their solubility in polar and non-polar molecules (5). In addition to illustrating enzyme activity the oxidation-reduction reaction also demonstrated enzyme specificity and enzyme-
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This note was uploaded on 10/01/2011 for the course BIO 012 taught by Professor Denberg during the Fall '08 term at University of Iowa.

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Bio Lab report 1 - I nvestigating enzyme properties in h...

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