I-LAB Examples _1 and _2BNS 2.24.11

I-LAB Examples _1 and _2BNS 2.24.11 - EcoRI/HpaIII...

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I-LAB PROBLEM #1 Watch the Example of I-Lab Do Problem #1 listed in your procedures Do this as a class and discuss RUN I-LAB EXAMPLE NOW Fill in Data Table as you go
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Data Collection Table
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I-LAB Molecular Weight LAMBDA MARKER GRAPH Lambda Molecular Weight Distance (mm) MW 1 23,130 95 9,416 1 6,557 2 4,361 3 2,322 4 2,027 155 564
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Distances Migrated (mm) * Note. . Must convert to cm for graphing RsaI 130, 141 AflIII 120 136, 141, 158 I-Lab Problem #1 Plasmid: PJAY1 Enzymes: RsaI, AflIII 1. GRAPH the MW vs Distance of the Lambda marker 2. Determine MW of each fragment 3. Fill in Data Table on next slide
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I-LAB PROBLEM #1 DATA TABLE RsaI AflIII Double 1,750 2,800 1,300 1,050 1,050 450 Total: 2,800 base pairs Estimated MW Taken from Graph
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PROBLEM #1 – PLASMID MAP RsaI AflIII RsaI 1,300 1,050 450
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PRACTICE PROBLEM 1 Draw a circular plasmid map for the following data set BglIII EcoRI HpaIII BglIII/EcoRI
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Unformatted text preview: EcoRI/HpaIII HpaIII/BglIII 7950 7950 7950 5416 4098 6632 2534 3852 1318 I-LAB PROBLEM #2 This is a practice problem to understand Restriction Mapping. Go through the assignment on I-lab and check your answers here. Distances Migrated (mm) FILL in with class: HincII _____ _____ BalI _____ Double Digest _____ ______ I-Lab Problem #2 Plasmid: PJAY2 Enzymes: HincIII, BalI 1. GRAPH the MW vs Distance 2. Determine MW of each fragment 3. Fill in Data Table on next slide I-LAB PROBLEM #2 DATA TABLE HincII BalI Double ____ ___ ____ ____ ___ ____ ____ Total:________ Estimated MW Taken from Graph I-LAB PROBLEM #2 - ANSWER Distance & Molecular Weight HincII 118mm: 3,100 bp 141mm: 1,050bp BalI 112mm: 4,200bp HincII & BalI 124mm: 2,400bp 141mm: 1,050bp 148mm: 720bp HincII BalI HincII 720 1,050 2,400...
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This note was uploaded on 10/01/2011 for the course BIOL 313L taught by Professor Kingston during the Fall '08 term at Iowa State.

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I-LAB Examples _1 and _2BNS 2.24.11 - EcoRI/HpaIII...

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