Part 2 - Recombinant DNA (Lab) BNS 2.24.11

Part 2 - Recombinant DNA (Lab) BNS 2.24.11 - Which one is...

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Split into groups 1. Concentration of DNA with nanodrop 2. Discussion Questions & Data Sheet
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Discussion 1. What was your yield? Was it consistent with others? 2. You have two DNA samples. A260 of 0.012 with an A260/A280 of 1.9. A260 of 0.851 with an A260/A280 of 1.2. Which is the purer sample? Which is the more concentrated sample?
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Unformatted text preview: Which one is better to use? 3. Work on data sheet #1-4. Restriction Enzyme Digestions Set-up Restriction Enzyme Digestions Add 10 ul of DNA to each tube of master mix Keep on ice Incubate tubes #1, #2 & #3 at 37 Remove the digestions after 3 hours...
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This note was uploaded on 10/01/2011 for the course BIOL 313L taught by Professor Kingston during the Fall '08 term at Iowa State.

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Part 2 - Recombinant DNA (Lab) BNS 2.24.11 - Which one is...

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