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Unformatted text preview: Transfer 700 µL of supernatant to spin column Centrifuge for 30 sec. & discard the flow-through Add 750 µL of Buffer PE Spin for 30 sec. & discard flow-through Centrifuge: 1 min. to remove residual wash buffer Cut lid off 1.5 mL microfuge tube and place spin column Add 50 µL sterile water to center of column to elute DNA REMINDERS Turn in tube to TA – Label Well Clean-up bench Throw E.coli cultures in WASTE TUBS...
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