lab report 2 final - Broadus, 1 Erinn Broadus Biology 104...

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Broadus, 1 Erinn Broadus Biology 104 Alyssa Underwood September 19, 2011 erinn.broadus@gmail.com Photosynthesis of Spinach Definition of Terms The lab was implemented to document the effects of varying levels of Sodium bicarbon- ate and differing light mechanisms and the effects that those variables have on photo- synthesis. 1. Photosynthesis is the process by which plants transform light energy into oxygen and through the calvin cycle, to sugars. The formula for photosynthesis is; carbon dioxide + water + light = glucose + water + oxygen. (Underwood, Pg. 3-9) 2. The sunlight is absorbed by the chlorophyll molecules. These molecules are found in the thylakoid of the chloroplast. (Hoffman) 3. The stomata are tiny openings on the leaf that allow for the transfer of gasses. (Hoff- man, pg. 32) It is through the stomata that the leaf gets carbon dioxide from the environ- ment and releases oxygen. Hypothesis 1. The spinach exposed to the lamp will have the most floating discs because room light and dark settings will not provide amble light energy for photosynthesis to take place. 2. The spinach exposed to the most sodium bicarbonate will have the most floating disks because the plant will utilize the extra carbon dioxide and turn it into oxygen. Materials and Methods First we set up a lamp so it was approximately 25 cm from the base. Then we filled a large beaker with 600 mL of cold water to absorb the heat admitted from the lamp. Three petri dishes were then filled 2/3 full with the NaHCO3 solution. Then ap- proximately 100 ml of the solution was filled into a 250 mL flask. Then 40-50 disks were cut out of chilled spinach leaves with a cork borer and the disks were then put into the flask. A water aspirator was then used to sink the disks by sucked out all of the oxygen.
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This note was uploaded on 10/03/2011 for the course BIO 104 taught by Professor Arnoldkarpoff during the Spring '08 term at University of Louisville.

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lab report 2 final - Broadus, 1 Erinn Broadus Biology 104...

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