Post Lab - February 17 - Lab#2 UV-V Absorbance spectrometer...

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
February 17, 2011 Lab #2 UV-V Absorbance spectrometer and Noise and Detection Limits Objective The Varian UV-Vis spectrometry is a scanning instrument that disperses the wavelengths of electromagnetic radiation across an entrance slit, sending only a narrow range of wavelengths through the sample and to a detector. Using the UV-Vis absorbance spectrometer, one will be able to gain experience observing the behavior of noise and the estimation of detection limits. To do so, a blank sample is placed into the sample compartment of the instrument recording P 0 (the absorbance of the blank sample at the selected wavelengths). The blank is then replaced with a sample and P is recorded at all wavelengths. The machine then plots absorbance versus wavelength (spectrum). This technique will be used to determine the upper limit for absorbance measurements. The lamp noise of the absorbance spectrometer will also be observed in order to calculate its detection limit.
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern