Quinine Determination - CHEM 4128 April 19, 2011...

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CHEM 4128 April 19, 2011 Determination of Quinine in Tonic Water by Fluorescence Objective Fluorescence is the emission of light by a substance that has absorbed electromagnetic radiation of a different wavelength . The emitted light has a longer wavelength, and, therefore lower energy than the absorbed radiation . In fluorescence measurement, the emission intensity F is proportional to the incident power, P 0 , of the excitations source, as well as the molar absorptivity ε and the concentration с . The following equation is used to measure the emission intensity: F = P 0 Ώεс Equation 1 The factor Ώ represents several conditions including the quantum yield and the collection and detection efficiencies . When the sample is concentrated, the power transmitting through the samples decreases exponentially with с and changes with F in accordance with Beer’s Law . In this lab we will be studying a component of Tonic water Quinine . Quinine fluoresces quite strongly and therefore can be detected at very low concentrations
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Quinine Determination - CHEM 4128 April 19, 2011...

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