In this experiment using catalase, we were trying to determine the average rate of a biochemical reaction using reaction tubes with assay solution inside. We used a reaction tube to rally 0.3 mL of a mix of diluted catalase and enzyme cocktail [hydrogen peroxide] into 6 other tubes all containing 3 mL of assay solution [iodine] at 30 second intervals. The longer we waited to put the reaction solution into the 6 tubes, the more the amount of hydrogen peroxide decreased producing a lighter brown-red iodine color, signifying the interaction of peroxide and iodine. We then measured absorbency levels of each tube at a wavelength of 500 nm in the spectrophotometer, and did the math to find peroxide levels of each tube. The tube with the largest hydrogen peroxide level was of course at 0.5 minutes [30 seconds], at about 0.0017 moles of hydrogen peroxide, followed by 1 minute at 0.0016 moles, 1.5 minutes [90 seconds], at 0.0014 moles, 2 minutes at 0.0013 moles, 2.5 minutes [150 seconds], at 0.0011 moles, and lastly 3
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This note was uploaded on 10/02/2011 for the course CHEM 210 taught by Professor Clay,m during the Summer '08 term at San Mateo Colleges.