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Unformatted text preview: 2) lacZ- encodes for beta galactosidase-hydrolyzes sugar lactose and synthetic molecular mimic called X-gal . [blue] 2) Plasmid and human DNA are restricted.-Plasmid is cut in the lacZ gene.-Human DNA is cut at many sites.-Each has sticky ends. 3) Mix human DNA fragments with cut plasmids-They base pair [complementary]-DNA ligase seals all fragments together.-Recombinants are created.-Not all DNA is recombinant. 4) The recombinant DNA above is mixed with bacteraia that has a mutation in own lacZ gene making them unable to hydrolize lactose as well.-Cells take up foreign DNA by transformation-Cells are various types of recombinants. 5) Bacteria is placed in solid agar that has ampicillin and X gal so that those with ampR gene will grow. Those would be plasmids. If cells are blue, then those are eliminated, for the blue cells have the lacZ gene and were not cut properly [also plasmids]....
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This note was uploaded on 10/02/2011 for the course CHEM 210 taught by Professor Clay,m during the Summer '08 term at San Mateo Colleges.
- Summer '08