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Lambda EcoRI-HindIII - Description Lambda DNA was...

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CERTIFICATE OF ANALYSIS Lambda Dglyph1197A/EcoRI+HindIII Marker, 3 #SM0191 250 (5x50) μg (for 500 applications) Lot: _ Concentration: 0.5 μg/μl Supplied with: 2x1 ml 6X DNA Loading Dye Store at -20°C. In total 7 vials. Description Lambda DNA was completely digested with EcoRI and HindIII, purified and dissolved in a storage buffer. The DNA marker contains the following 13 discrete fragments (in base pairs): 21226 *, 5148, 4973, 4268, 3530 *, 2027, 1904, 1584, 1375, 947, 831, 564, 125. Storage Buffer 10 mM Tris-HCl (pH 7.6), 1 mM EDTA. 6X DNA Loading Dye 10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA. Quality Control Assay Data Well-defined bands are formed during agarose gel electrophoresis. The DNA concentration is determined spectrophptometrically. The absence of nucleases is confirmed by a direct nuclease activity assay. Quality authorized by: Jurgita Zilinskiene Rev V
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RECOMMENDATIONS FOR USE Vortex gently just prior to use. Prepare the DNA marker before loading: 1 μl (0.5 μg) of the DNA marker; 1 μl of 6X DNA Loading Dye; 4 μl of deionized water.
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