Lambda EcoRI-HindIII - Description Lambda DNA was...

CERTIFICATE OF ANALYSIS
Lambda Dglyph1197A/EcoRI+HindIII
Marker, 3
#SM0191 250 (5x50) μg
(for 500 applications)
Lot: _
Concentration: 0.5 μg/μl
Supplied with: 2x1 ml 6X DNA Loading Dye
Store at -20°C.
In total 7 vials.
Description
Lambda DNA was completely digested with EcoRI and
HindIII, purified and dissolved in a storage buffer.
The DNA marker contains the following 13 discrete
fragments (in base pairs): 21226 *, 5148, 4973, 4268,
3530 *, 2027, 1904, 1584, 1375, 947, 831, 564, 125.
Storage Buffer
10 mM Tris-HCl (pH 7.6), 1 mM EDTA.
6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue,
0.03% xylene cyanol FF, 60% glycerol and
60 mM EDTA.
Quality Control Assay Data
Well-defined bands are formed during agarose gel
electrophoresis.
The DNA concentration is determined
spectrophptometrically.
The absence of nucleases is confirmed by a direct
nuclease activity assay.
Quality authorized by:
Jurgita Zilinskiene
Rev
V

RECOMMENDATIONS FOR USE
• Vortex gently just prior to use.
• Prepare the DNA marker before loading:
1 μl (0.5 μg) of the DNA marker;
1 μl of 6X DNA Loading Dye;
4 μl of deionized water.