This preview has intentionally blurred sections. Sign up to view the full version.View Full Document
Unformatted text preview: CERTIFICATE OF ANALYSIS Lambda D&A/HindIII Marker, 2 #SM0101 250 (5x50) μg Lot: _ Concentration: 0.5 μg/μl Supplied with: 2x1 ml 6X DNA Loading Dye Store at -20°C . In total 7 vials. Description Lambda DNA was completely digested with HindIII, purified and dissolved in a storage buffer. The DNA marker contains the following 8 discrete fragments (in base pairs): 23130*, 9416, 6557, 4361*, 2322, 2027, 564, 125. Storage Buffer 10 mM Tris-HCl (pH 7.6), 1 mM EDTA. 6X DNA Loading Dye 10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA. Quality Control Assay Data Well-defined bands are formed during agarose gel electrophoresis. The DNA concentration is determined spectrophptometrically. The absence of nucleases is confirmed by a direct nuclease activity assay. Quality authorized by: Jurgita Zilinskiene Rev V RECOMMENDATIONS FOR USE • Vortex gently just prior to use....
View Full Document
This note was uploaded on 10/03/2011 for the course CHEM 113A taught by Professor Professornotknown during the Spring '09 term at San Jose State.
- Spring '09