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Unformatted text preview: CERTIFICATE OF ANALYSIS FastDigest ® NsiI (Mph1103I) * #FD0734 100 μl (for 100 reactions) Lot: Expiry Date: 5' ... A T G C A T ... 3' 3' ... T A C G T A ... 5' *FastDigest ® NsiI (Mph1103I) is a proprietary formulation of Mph1103I, an isoschizomer of NsiI having the same recognition and cleavage specificity. Supplied with: 1 ml of 10X FastDigest ® Buffer 1 ml of 10X FastDigest ® Green Buffer Store at -20°C In total 3 vials. BSA included Description FastDigest ® enzymes are an advanced line of restriction enzymes for rapid DNA digestion. All FastDigest ® enzymes are 100% active in the universal FastDigest ® and FastDigest ® Green buffers and are able to digest DNA in 5-15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. FastDigest ® enzymes can be used to digest plasmid, genomic and viral DNA as well as PCR products and do not show star activity even in prolonged incubations. Enzymes used in common downstream applications such as ligation, blunting and dephosphorylation reactions also have 100% activity in FastDigest ® and FastDigest ® Green Buffer. FastDigest ® Green Buffer includes a density reagent along with blue and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The blue dye of the FastDigest ® Green Buffer migrates with 3-5 kb DNA fragments in a 1% agarose gel and has an excitation peak at 424 nm. The yellow dye of the FastDigest ® Green Buffer migrates faster than 10 bp DNA fragments in a 1% agarose gel and has an excitation peak at 615 nm....
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