pGEX - GE Healthcare pGEX vectors, GST Gene Fusion System...

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Unformatted text preview: GE Healthcare pGEX vectors, GST Gene Fusion System pGEX-1λT Thrombin Leu Val Pro Arg↓Gly Ser Pro Glu Phe Ile Val Thr Asp CTG GTT CCG CGT GGA TCC CCG GAA TTC ATC GTG ACT GAC TGA CGA Stop codons EcoRI BamHI pGEX-2T Thrombin Leu Val Pro Arg↓Gly Ser Pro Gly Ile His Arg Asp CTG GTT CCG CGT GGA TCC CCG GGA ATT CAT CGT GAC TGA CTG ACG Stop codons BamHI SmaI EcoRI pGEX-2TK Thrombin Map of the glutathione S-transferase fusion vectors showing reading frames and main features. Even though stop codons in all three frames are not depicted in this map, all thirteen vectors have stop codons in all three frames downstream from the multiple cloning site. Kinase Leu Val Pro Arg↓ Gly Ser Arg Arg Ala Ser Val CTG GTT CCG CGT GGA TCT CGT CGT GCA TCT GTT GGA TCC CCG GGA ATT CAT CGT GAC TGA Stop codons BamHI SmaI EcoRI pGEX-4T-1 Thrombin Leu Val Pro Arg ↓Gly Ser Pro Glu Phe Pro Gly Arg Leu Glu Arg Pro His Arg Asp CTG GTT CCG CGT GGA TCC CCG GAA TTC CCG GGT CGA CTC GAG CGG CCG CAT CGT GAC TGA Stop codons NotI EcoRI SmaI SalI XhoI BamHI pGEX-4T-2 Thrombin Leu Val Pro Arg ↓Gly Ser Pro Gly Ile Pro Gly Ser Thr Arg Ala Ala Ala Ser CTG GTT CCG CGT GGA TCC CCA GGA ATT CCC GGG TCG ACT CGA GCG GCC GCA TCG TGA Stop codon BamHI NotI EcoRI SmaI SalI XhoI pGEX-4T-3 Ordering information Thrombin Leu Val Pro Arg↓ Gly Ser Pro Asn Ser Arg Val Asp Ser Ser Gly Arg Ile Val Thr Asp CTG GTT CCG CGT GGA TCC CCG AAT TCC CGG GTC GAC TCG AGC GGC CGC ATC GTG ACT GAC TGA Stop codons BamHI NotI EcoRI SmaI SalI XhoI Product Quantity Code no. pGEX-1λT EcoRI/BAP 5 µg 28-9546-56 pGEX-3X pGEX-2T 25 µg 28-9546-53 pGEX-2TK 25 µg 28-9546-46 Ile Glu Gly Arg ↓Gly Ile Pro Gly Asn Ser Ser ATC GAA GGT CGT GGG ATC CCC GGG AAT TCA TCG TGA CTG ACT GAC Stop codons BamHI SmaI EcoRI pGEX-3X 25 µg 28-9546-54 pGEX-4T-1 25 µg 28-9545-49 pGEX-4T-2 25 µg 28-9545-50 pGEX-4T-3 25 µg 28-9545-52 pGEX-5X-1 25 µg 28-9545-53 pGEX-5X-2 25 µg 28-9545-54 pGEX-5X-3 25 µg 28-9545-55 pGEX-6P-1 25 µg 28-9546-48 pGEX-6P-2 25 µg 28-9546-50 pGEX-6P-3 25 µg 28-9546-51 Do you want to learn more? Read the GST Gene Fusion System Handbook (18-1142-75). Please contact your local GE Healthcare representative for a printed copy. Factor Xa pGEX-5X-1 Factor Xa Ile Glu Gly Arg ↓Gly Ile Pro Glu Phe Pro Gly Arg Leu Glu Arg Pro His Arg Asp ATC GAA GGT CGT GGG ATC CCC GAA TTC CCG GGT CGA CTC GAG CGG CCG CAT CGT GAC TGA Stop codons BamHI SalI XhoI NotI EcoRI SmaI pGEX-5X-2 Factor Xa Ile Glu Gly Arg ↓Gly Ile Pro Gly Ile Pro Gly Ser Thr Arg Ala Ala Ala Ser ATC GAA GGT CGT GGG ATC CCC GGA ATT CCC GGG TCG ACT CGA GCG GCC GCA TCG TGA Stop codon SalI XhoI BamHI NotI EcoRI SmaI pGEX-5X-3 Factor Xa Ile Glu Gly Arg ↓ Gly Ile Pro Arg Asn Ser Arg Val Asp Ser Ser Gly Arg Ile Val Thr Asp ATC GAA GGT CGT GGG ATC CCC AGG AAT TCC CGG GTC GAC TCG AGC GGC CGC ATC GTG ACT GAC TGA Stop codons EcoRI SmaI SalI XhoI BamHI NotI pGEX-6P-1 PreScission™ Protease Leu Glu Val Leu Phe Gln ↓Gly Pro Leu Gly Ser Pro Glu Phe Pro Gly Arg Leu Glu Arg Pro His CTG GAA GTT CTG TTC CAG GGG CCC CTG GGA TCC CCG GAA TTC CCG GGT CGA CTC GAG CGG CCG CAT BamHI EcoRI pGEX-6P-2 SmaI SalI XhoI PreScission Protease Leu Glu Val Leu Phe Gln↓ Gly Pro Leu Gly Ser Pro Gly Ile Pro Gly Ser Thr Arg Ala Ala Ala Ser CTG GAA GTT CTG TTC CAG GGG CCC CTG GGA TCC CCA GGA ATT CCC GGG TCG ACT CGA GCG GCC GCA TCG BamHI EcoRI SmaI pGEX-6P-3 SalI XhoI NotI PreScission Protease Leu Glu Val Leu Phe Gln↓Gly Pro Leu Gly Ser Pro Asn Ser Arg Val Asp Ser Ser Gly Arg CTG GAA GTT CTG TTC CAG GGG CCC CTG GGA TCC CCG AAT TCC CGG GTC GAC TCG AGC GGC CGC BamHI EcoRI SmaI SalI NotI XhoI BalI h Tth111I AatII ase nsfer -tra eS ion gl at ut Ptac p Am BspMI r pSj10 Bam7Stop7 PstI pGEX ~4900 bp NarI EcoRV imagination at work NotI la AlwNI p4.5 cq I BssHII ApaI BstEII MluI pBR322 ori For local office contact information, visit www.gelifesciences.com/contact www.gelifesciences.com/pgex GE Healthcare Bio-Sciences AB Björkgatan 30 751 84 Uppsala Sweden GE, imagination at work, and GE monogram are trademarks of General Electric Company. pGEX vectors are to be used for scientific investigation and research and for no other purpose whatsoever and a license for commercial use of the licensed products and the processes claimed in US patent 5,654,176 and equivalent patents and patent applications in other countries must be negotiated directly with Millipore Corp (formerly Chemicon International Inc) by the purchaser prior to such use. © 1993–2009 General Electric Company – All rights reserved. First published June 1993. All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare representative for the most current information. GE Healthcare UK Limited Amersham Place Little Chalfont, Buckinghamshire, HP7 9NA UK GE Healthcare Europe, GmbH Munzinger Strasse 5, D-79111 Freiburg Germany GE Healthcare Bio-Sciences Corp. 800 Centennial Avenue, P.O. Box 1327, Piscataway, NJ 08855-1327 USA GE Healthcare Bio-Sciences KK Sanken Bldg., 3-25-1, Hyakunincho, Shinjuku-ku, Tokyo 169-0073 Japan imagination at work 28-9191-62 AC 07/2009 ...
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This note was uploaded on 10/03/2011 for the course CHEM 113A taught by Professor Professornotknown during the Spring '09 term at San Jose State.

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