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CERTIFICATE OF ANALYSIS Hot Start Taq DNA Polymerase #EP0601 100 u Lot: _ Expiry Date: _ Concentration: 5 u/µl Supplied with: 0.6 ml of 10X Hot Start PCR Buffer 0.6 ml of 25 mM MgCl 2 Store at -20°C Description Maxima ® Hot Start Taq DNA Polymerase is designed to enhance the specificity, sensitivity and yield of DNA amplification (1-4). In addition, the enzyme provides the convenience of reaction set-up at room temperature. Maxima ® Hot Start Taq DNA Polymerase is a recombinant Taq DNA polymerase which has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. The functional activity of the enzyme is restored during a short 4-minute incubation at 95°C. The activated enzyme maintains the same functionality as Taq DNA polymerase: catalyzes 5’ 3’ synthesis of DNA, has no detectable 3’ 5’ proofreading exonuclease activity, but possesses low 5’ 3’ exonuclease activity. It exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Before activation, the two activities are not detectable. Applications • Hot start PCR. • RT-PCR. • Highly specific amplification of complex genomic and cDNA templates up to 3 kb. • Amplification of low copy DNA targets. • Real-time PCR. • Multiplex PCR. • Generation of PCR products for TA cloning. Rev.9 V
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Source E. coli cells carrying a cloned pol gene from Thermus ± aquaticus. ± Definition of Activity Unit One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 min at 74°C.
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