Wizard SV SV Genomic DNA Purification System Protocol

Wizard SV SV Genomic DNA Purification System Protocol -...

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Technical Bulletin Wizard ® SV Genomic DNA Purification System INSTRUCTIONS FOR USE OF PRODUCTS A2360, A2361 AND A2365. PRINTED IN USA. Revised 3/09 Part# TB302
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Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TB302 Revised 3/09 Page 1 1. Description .......................................................................................................... 1 2. Product Components ......................................................................................... 3 3. Isolation of Genomic DNA from Mouse Tail Clippings or Animal Tissues .............................................................................................. 4 A. Preparation of Solutions. .................................................................................................. 4 B. Preparation of Mouse Tail and Tissue Lysates . ........................................................... 5 C. Purification of Genomic DNA from Lysates Using a Microcentrifuge. .................. 6 D. Purification of Genomic DNA from Lysates Using a Vac-Man ® Vacuum Manifold . ............................................................................................................................. 7 4. Isolation of Genomic DNA from Tissue Culture Cells ............................. 8 A. Preparation of Solutions. .................................................................................................. 8 B. Preparation of Tissue Culture Cell Lysates . ................................................................. 8 C. Purification of Genomic DNA from Tissue Culture Cell Lysates Using a Microcentrifuge. ............................................................ 9 D. Purification of Genomic DNA from Tissue Culture Cell Lysates Using a Vac-Man ® Vacuum Manifold. ........................................................................ 10 5. Troubleshooting ............................................................................................... 12 6. Related Products ............................................................................................... 15 1. Description The Wizard ® SV Genomic DNA Purification System (a) provides a fast, simple technique for the preparation of purified and intact DNA from mouse tails, tissues and cultured cells in as little as 20 minutes, depending on the number of samples processed. For this system, either a spin or vacuum (“SV”) purification protocol can be used (Figure 1). Up to 20mg of tissue (mouse tail or animal tissue) or between 1 × 10 4 and 5 × 10 6 tissue culture cells can be processed per purification. The genomic DNA isolated with this system is of high quality and serves as an excellent template for agarose gel analysis, restriction enzyme digestion and PCR analysis. Table 1 provides typical yields of genomic DNA purified from a variety of sources. Wizard
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Wizard SV SV Genomic DNA Purification System Protocol -...

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