02. Microscopy

02. Microscopy - Lab 2 Page 1 Lab 2 Lab Clean-Up (Page 2)...

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Unformatted text preview: Lab 2 Page 1 Lab 2 Lab Clean-Up (Page 2) Clean-up is an important Aspect of Lab Safety. Today youll have lots of Slides for the Metal Pitcher and lots of Plates for Oscar. Lab Exercises 1- Zeiss Microscope Nomenclature (Pages 3 and 4) 2- Zeiss Microscope Brightfield Alignment (Pages 5-8) Remember the first Time you tried to ride a Multi-Speed Bicycle with Hand Brakes? It looked impossible! The Zeiss Microscopes we use in MIC 101 are likewise a bit intimidating at first. Its just another Machine. Please do not hesitate to ask Questions!. 3- Gram Staining (Pages 9-12) 4- Viewing a Smear (Page 13) 5- Oil Immersion (Page 14) 6- Photomicroscopy (Page 15) You made Smears last Week and your TA and Tutor picked the best Smears for Gram Staining. Today youll make Smears and do the Gram Staining. Then youll look at them using the 10X and 40X Lenses. But to really call a Gram Stain youll need to use your 100X Lens and that requires Oil Immersion. You can take Pictures through your Microscope using your Digital Camera. Its amazingly simple. 7- Cleaning and Storing your Microscope (Page 16) Microscope Clean-up tends to be a frequent Exit Visa. Twelve different Students are using each Microscope so it is really important that you (and they) clean it up and store it in its proper Parking Spot in the Microscope Cabinet. 8- Streaking (Pages 17 and 18) Check your Streak Plates of Serratia and Bacillus from the last Lab You should be able to make-out individual Red Colonies on at least one of your Serratia Streak Plates and individual Yellow Colonies on at least one of your Bacillus Plates. If not, dont Panic. Youll get the hang of Streaking after a few more Practices. Background Information for Lab 2 Exercises (Pages 19-21) Lab 2 Page 2 Lab 2 Clean-Up Once again the Basic Rules are: ! If it ! s Sharp Glass (Slides, Cover Slips) - Put it in the Metal Pitcher ! If it ! s a Petri Plate - Put it in Oscar ! If it ! s Reusable Glassware (Test Tubes, Flasks, Bottles) - Put it in the Aluminum Bus Pan after you ! ve removed any Tape or Labels ! If it ! s a Serological Pipette- Put it in the Plastic Tray after you ! ve removed the Cotton Plug with your bent Paper Clip The Specifics for Todays Lab are: All Slides go into the Metal Pitcher - You can simply dump the Contents of your Dissecting Dish (Germicidal 64 and all) into the Metal Pitcher Petri Plates go to Oscar - Oscar likes Tape so theres no need to remove it Please clean-up and put away your Microscope - Please remove your Slide from the Stage - Please remove the Immersion Oil from the 100X Lens - Please wrap the Cord around your Microscope Lariat Style (High-Ho, Silver! Away!) -- or like your Vacuum Cleaner if youre not at Home on the Range -- and return it to its Reserved Spot in the Microscope Cabinet Please swab-down your Bench Top with Germicidal 64 before you leave - And please dont forget to wash your Hands Lab 2 Page 3...
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02. Microscopy - Lab 2 Page 1 Lab 2 Lab Clean-Up (Page 2)...

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