08. Biochemical Testing

08. Biochemical Testing - Lab 8 Lab Clean-Up Oh weve got...

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Lab 8 Page 1 Lab 8 Lab Clean-Up Oh, we’ve got beaucoup Stuff for Today’s Lab Clean-Up. Once again it’d be a Most Excellent Idea to clean-up as you go instead of waiting until the End. Lab Exercises 1- Viable Count Results (Page 3) It’s another Opportunity to be The Count (“I am The Count: 1, 2, 3. ..”) so you can count the Number of Colonies on your Plates. Use the Clickers or be Stupid. Then, just like with the Phage Titer Experiment, you back-calculate the Number of Bacteria per Gram of Hamburger. 2- Water Quality Testing Results (Pages 4 and 5) This is insanely simple. You inoculated 3 Sets of Tubes. Now count the Number of Tubes within each Set that are Turbid (i.e. Positive). This will result in a 3 Digit Number where each Digit can be a 0, 1, 2 or 3 (i.e. 3-3-1, 3-2-1, 3-2-2). Look up this Number on the MPN Chart on Page 5. That’s it. Just remember that MPN is the Number of Coliform Bacteria per 100 ml. 3- Biochemical Tests (Pages 6-11) Today you’ll begin using a Series of Biochemical Tests to identify your Isolates. You’ll be able to complete some of these Tests; others you’ll only be able to set-up and “read” in the next Lab. You are going to be using lots and lots of Tubes so as with Water Quality Testing, careful Labeling and good Communication with your Lab Partner are really important. 4- Biochemical Unknowns (Page 12) Biochemical Unknowns are Open Book. Each Clan will sign-up for an Unknown Bacterium (which is one of the seven Bacteria you first saw in our Rogues Gallery in Lab 1). You should perform Gram Stains, then you should proceed with the appropriate Biochemical Tests. Background Information for Lab 8 Exercises (Pages 15-23)
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Lab 8 Page 2 Lab 8 Clean-Up We’ve got a Butt-load Stuff that needs to be disposed of properly. Ideally you will have been doing this as you work, but just in case, here’s the Big Picture. Viable Count • Plates go to Oscar - There’s no Need to remove any Tape Water Quality Testing LSL Tubes (in Cardboard Containers) • Please remove the Tape from all your Tubes • Place your Tubes (still in the Cardboard Container ) on the indicated Location on the Back Bench. - These Tubes reek so please don’t put them in the Metal Bus Pan Biochemical Testing and Biochemical Unknowns • Slides go into the Metal Pitcher • Used Toothpicks go into the large Waste Beaker • Unused Toothpicks (still in the Beaker) go back into the Basket from which you took them. - We can autoclave these guys and re-use them. • Filter Paper goes to Oscar - It’s contaminated with Bacteria You can once again do us a huge Favor by clearing-out any of your Plates, Tubes, or Flasks in the any of the Incubator Bins, 谢谢 ! Xie-Xie!
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Lab 8 Page 3 Lab 8 Exercises Viable Counts (Atlas of Microbiology, Page 83) 1. Look at all of your Plates but check for Plates with between 30 and 300 Colonies. Colonies can overlap and still be counted.
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08. Biochemical Testing - Lab 8 Lab Clean-Up Oh weve got...

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