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Unformatted text preview: Lab 7 Page 1 Lab 7 Lab Clean-Up (Page 2) There are a lot of different Lab Exercises Today so there’s a lot of different Stuff that needs to be put into different Containers on the Waste Cart. Like an efficient Cook you should be continuously cleaning-up as you proceed. Lab Exercises 1- Viable Count (Pages 3-5) A Viable Count, as the Name implies, tells you approximately how many living (“viable”) Cells you have in a given Volume. The Viable Count Exercise is fundamentally similar to the Bacteriophage Titer Exercise. 2- Water Quality Testing (Pages 6 and 7) The Most Probable Count, as the Name implies, is a statistical Determination of the Number of Coliform Bacteria per 100 ml of a Fluid (usually Water). 3- Phage Titer Results (Page 8) The Phage Titer Exercise allows you to determine the Number of Phage in the initial Phage Suspension we gave you two Labs back. 4- Antibiotic Results (Page 9) The Antibiotic Testing Exercise allows you to determine “Sensitivity” or “Resistance” of E. coli and Staphylococcus epidermidis to several Antibiotics. 5- Isolations (Pages 10-13) Isolations will once again involve making Gram Stains, measuring the stained Bacteria, and then streaking a new Plate from the same Colony you used to make the Gram Stain. 6- Hemocytometer (Page 14) Hemocytometers, as the Name implies, are Devices that can be used to determine the Number of Cell-Size Entities in a given Volume of Fluid. Background Information for Lab 7 Exercises (Pages 15-20) Lab 7 Page 2 Lab 7 Clean-Up We’ve got a Butt-load Stuff so you’re all going to have to be Lady Kenmore. Ideally you will have been doing this as you work, but just in case, here’s the Big Picture. Viable Count • Test Tubes go into the Metal Buspan - Please remove all Tape - Please remove the Caps and put them in the Wire Basket - Please orient your Test Tubes in the same Direction • Microtiter Pipette Tips go into the labeled Waste Beaker on the Back Bench • Hockey Sticks go into the Metal Pitcher • Ethanol Beakers go back on the Media Shelves by the Windows • Serological Pipettes go into the Plastic Tray - Please remove the Cotton Plugs Water Quality Testing • Microtiter Pipette Tips go into the labeled Waste Beaker on the Back Bench • Flasks go into the Metal Bus Pan • Please remove the Aluminum Foil (put it in Oscar) • Serological Pipettes go into the Plastic Tray - Please remove the Cotton Plugs Phage Titer , Antibiotic Testing , Isolations • Petri Plates go to Oscar Gram Stain • All Slides go into the Metal Pitcher Hemocytometer • Microtiter Pipette Tips go into the labeled Waste Beaker on the Back Bench • Please carefully rinse your Hemocytometer and its special Cover Slip with DI Water - Blot both dry and return them to their ratty red Plastic Containers You can do us a Big Favor by clearing-out any of your Plates, Tubes, or Flasks in the 37°C Incubator Bins, 30°C Incubator Bins or 04°C (Cold Room) Bins....
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- Spring '09
- Microbiology, Escherichia coli, gram-negative bacteria, Agar plate, Gram-positive bacteria