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Unformatted text preview: Lab 4 Page 1 Lab 4 Lab Clean-Up (Page 2) There’ll be Pipettes in the Plastic Tray, Pipette Tips in the Labeled Flask on the Bench by the Cart, Petri Plates for Oscar and lots of Slides for the Metal Pitcher. Lab Exercises 1- Serological Pipettes and Microtiter Pipettes (Pages 3-6) Watch carefully while your Lab Instructor and Tutor demonstrate how to use our profoundly annoying Serological Pipettes and how to use our spiffy Microtiter Pipettes. Both are really, really easy to master. 2- Growth Curve (Pages 7-11) You’ll be doing the Growth Curve Experiment as a Clan. Be sure and know who is doing what, and regardless of what you’re doing, be sure and know the entire Experiment. 3- Isolations (Pages 12 and 13) We’re going to do Gram Stains on your putative (L= thought to be) Bacillus , Staphylococcus and Enterics. We’ll also streak-out your Sodium Benzoate Broth to get Pseudomonas Colonies. All this Streaking and Smearing and Staining not only continues the Isolations but gives you lots and lots of useful Practice. 4- Brightfield Microscopy Alignment (Pages 14 and 14) Don’t worry; it will all come back to you surprisingly fast after a Review. 5- Gram Stains (Page 15) Up to now, Gram Stains (like Streaking) have simply been an abstract Exercise. But starting Today Gram Stains will become another Technique for identifying your Isolates. 6- Spore Stain (Pages 18 and 19) Remember when we talked about Endospores in Lecture? They’re these incredibly resistant “Life Boats” or “Survival Pods” that some Bacteria -- particularly Bacillus and Clostridium can produce. How do you get Stain into such a resistant Structure? You cook them! It’s really quite simple. Background Information for Lab 4 Exercises (Pages 21-33) Lab 4 Page 2 Lab 4 Clean-Up The Basic Rules are: If it ʼ s Sharp Glass (Slides, Cover Slips) - Put it in the Metal Pitcher If it ʼ s a Petri Plate - Put it in Oscar If it ʼ s Reusable Glassware (Test Tubes, Flasks, Bottles) - Put it in the Aluminum Bus Pan after you ʼ ve removed any Tape or Labels If it ʼ s a Serological Pipette- Put it in the Plastic Tray after you ʼ ve removed the Cotton Plug with your bent Paper Clip The Specifics for Today’s Lab are: • Used Serological Pipettes are placed in a large Nalgene Pipette Jar on your Bench during the Lab Period. When you are done for the day, please remove the Cotton Plugs from your Pipettes (very important), discard the Plugs and place your Pipettes in the Plastic Pipette Bin with the Tips all in the same Orientation (an inscrutable Metal Placard indicates this Orientation). • Gram Stain and Spore Stain Slides go into the Metal Pitcher • Petri Plates go to Oscar • Please swab-down your Bench Top with Germicidal 64 before you leave - And don’t forget to wash your Hands Remember to save all of your Isolates: • Pseudomonas Tubes go into Cardboard Cups in 30°C Bins • Bacillus Nutrient Agar Plates go into 04°C Bins • Staphylococcus YGC Plates go into 04°C Bins • Enteric EMB Plates go into 04°C Bins Lab 4 Page 3...
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This note was uploaded on 10/03/2011 for the course MIC 101 taught by Professor Mann during the Spring '09 term at UC Davis.
- Spring '09