03. Isolations

03. Isolations - Lab 3 Lab Clean-Up (Page 2) Youll have...

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Lab 3 Page 1 Lab 3 Lab Clean-Up (Page 2) You’ll have lots of Slides and Cover Slips for the Metal Pitcher, Plates for Oscar and Test Tubes for the Metal Bus Pan. Lab Exercises 1- Isolations (Pages 3 and 4) In Today’s Lab you’ll start a several Week Project to isolate and obtain Pure Cultures of Pseudomonas sp. and Bacillus sp. from Soil Samples, and Staphylococcus sp. and Enteric Bacteria (E. coli and/or Enterobacter sp.) from “Patient” Samples. 2- Review of Microscope Nomenclature (Page 5) It’s been a Week since you last saw your Microscope so we’ll go over all the Parts again. 3- Review of Brightfield Alignment (Page 6) It’s been a Week since you last saw your Microscope so. .. 5- Negative Staining (Page 7) Negative Staining is used to show Capsules (which are [usually] Layers of Carbohydrate outside the Cell Wall that sponge-up enormous Amounts of Water and make the Bacterium slippery and difficult to phagocytose). 4- Gram Staining (Page 8) It’s been a Week since you last did a Gram Stain so. .. 6- Ocular Micrometer Calibration (Page 9) We’ll use Stage Micrometers with very precise, 100 Micron Lines to determine how many Microns each Ocular Unit of the Ocular Micrometer (in your Left Eyepiece) represents at each Magnification. 7- Streaking (Page 10) Up to now, Streaking simply been an abstract Exercise. But starting Today Streaking becomes a Technique for selecting and maintaining your Isolates. Background Information for Lab 3 Exercises (Pages 11-22)
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Lab 3 Page 2 Lab 3 Clean-Up The Basic Rules are: If it ʼ s Sharp Glass (Slides, Cover Slips) - Put it in the Metal Pitcher If it ʼ s a Petri Plate - Put it in Oscar If it ʼ s Reusable Glassware (Test Tubes, Flasks, Bottles) - Put it in the Aluminum Bus Pan after you ʼ ve removed any Tape or Labels If it ʼ s a Serological Pipette - Put it in the Plastic Tray after you ʼ ve removed the Cotton Plug with your bent Paper Clip The Specifics for Today’s Lab are: • Slides and Cover Slips go into the Metal Pitcher • Petri Plates go to Oscar - There is no need to remove any Tape - Please tell us when Oscar is getting Full • Test Tubes go into the Metal Buspan - Please remove all Tape from Test Tubes - Please remove the Caps and put them in the Wire Basket - Please orient the Tubes in the same Direction like Saturated Lipids • Please swab-down your Bench Top with Germicidal 64 before you leave - And don’t forget to wash your Hands with our Orange-Flavored GoJo
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Lab 3 Page 3 Lab 3 Exercises Isolations Isolation of Pseudomonas (Enrichment) Materials [per Student] • Sterile 16mm Test Tube • Sodium Benzoate Broth • Soil Sample Procedure 1. Swirl the Sodium Benzoate Broth and add ~5 ml (~ 3 Fingers) of this Sodium Benzoate Broth to a labeled 16mm Test Tube. 2.
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This note was uploaded on 10/03/2011 for the course MIC 101 taught by Professor Mann during the Spring '09 term at UC Davis.

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03. Isolations - Lab 3 Lab Clean-Up (Page 2) Youll have...

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