Exam2_Summer09_KEY - GENE 3200 – Summer 2009 – Bedell Exam 2 – June 29 ID no 810 FINAL KEY 1 ♦ Write your ID number(810 number NOT SSN on

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Unformatted text preview: GENE 3200 – Summer 2009 – Bedell Exam 2 – June 29 ID no. 810_________ FINAL KEY ___________ 1 ♦ Write your ID number (810 number, NOT SSN) on every page . ♦ Write your initials on the first page only . ♦ All of the multiple choice questions have only one correct answer. ♦ The exam should be completed in ink. Regrades will not be given if you write in pencil. ♦ All requests to re-grade an exam must be made in writing to Dr. Bedell within 48 hours of the grades being posted on WebCT. 1. In Drosophila, the following autosomal recessive mutations have been observed (where capital letters are used for dominant alleles and lower case letters are used for recessive alleles): Wildtype Mutant Body color grey (B) black (b) Wing shape long (D) dumpy (d) Bristle morphology unhooked (H) hooked (h) From a cross of a dumpy female with a black, hooked male, all of the F1 were wildtype for all three traits. The test cross of an F1 female with a dumpy, black, hooked male resulted in 1000 progeny in 8 phenotypic classes: I. wildtype 169 II. black 19 III. black, hooked 301 IV. dumpy, hooked 21 V. hooked 8 VI. hooked, dumpy, black 172 VII. dumpy, black 6 VIII. dumpy 304 1A. (4 pts). What are the map distances between the following pairs of genes? Show all equations for your calculations. B – D _________ 35.5 m.u ._______ B – H _________ 5.4 m.u. ________ D – H _________ 40.9 m.u ._________ D – B distance = (172 + 169 + 6 + 8)/1000 = 355/1000 = 35.5 m.u. B – H distance = (21 + 19 + 6 + 8)/1000 = 54/1000 = 5.4 m.u. D – H distance = (172 + 169 + 21 + 19 + 6 + 6 + 8 + 8)/1000 = 409/1000 = 40.9 m.u. or D – H distance = 35.5 + 5.4 = 40.9 m.u. 1B. (3 pts). How much crossover interference (if any) occurred. Show all equations for your calculations. Expected double crossovers = 0.355 x 0.054 x 1000 = 19 Interference = I = 1 – observed DCO = 1 - 14 = 1 – 0.737 = 0.263 (or 26.3%) expected DCO 19 0.26 (or 0.263) GENE 3200 – Summer 2009 – Bedell Exam 2 – June 29 ID no. 810_________ FINAL KEY ___________ 2 Questions 2 – 5 refer to the four structures shown below. 2. (1 pt) Which of the structures is guanine ? A, B, C , D, or none 3. (1 pt) Which of the structures is thymine ? A, B , C, D, or none 4. (1 pt) If any of the structures had a deoxyribose linked to it, what would it be called? A. purine D. nucleoside B. pyrimidine E. base C. nucleotide F. none of the above 5. (2.5 pts). If 35% of the bases in mouse DNA are G, what percentage of the bases are T? Show your calculations. If G = 0.35 the G + A = 0.70, So A + T = 0.30, A = T = 0.15 (or 15% 6. When Meselson and Stahl performed their experiments on DNA replication, they grew cultures of E. coli in media with different isotopes of nitrogen for one or two generations and then centrifuged the DNA from each culture in a CsCl gradient. The possible results of their experiment are shown in the three panels (A, B, and C) to the right, with dashed horizontal lines indicating the positions of DNA after...
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This note was uploaded on 10/05/2011 for the course GENE 3200 taught by Professor Condie during the Summer '08 term at University of Georgia Athens.

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Exam2_Summer09_KEY - GENE 3200 – Summer 2009 – Bedell Exam 2 – June 29 ID no 810 FINAL KEY 1 ♦ Write your ID number(810 number NOT SSN on

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