Exam3_Fall2010

Exam3_Fall2010 - GENE3200 Fall 2010 Bedell Exam 3 Nov 4 ID...

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GENE3200 – Fall 2010 – Bedell Exam 3 – Nov 4 ID no. 810 ______________________________ 1 Write your ID number (810 number, NOT SSN) on every page. Write your initials on the first page only. All of the multiple choice questions have only one correct answer. The exam should be completed in ink. Regrades will not be given if you write in pencil. All requests to re-grade an exam must be made in writing to Dr. Bedell within 48 hours of the grades being posted on WebCT. 1. (1 pt) True or false. To clone a 150 kb fragment of genomic DNA, the most appropriate cloning vector is a BAC. 2. (2 pts) An ideal plasmid used as a cloning vector in E. coli should have ______. (a) an ori sequence (b) a selectable marker (c) unique restriction sites (d) all of the above (e) none of the above 3. (2 pts) Which of the following statements is true of cDNA? (a) DNA that has been cloned into a vector. (b) DNA that is circularized. (c) DNA copied from RNA by reverse transcriptase. (d) All of the above (e) None of the above 4. (2 pts) In a human genomic DNA library made by complete SalI (GTCGAC) digestion, how many clones constitute a genomic equivalent? As a reminder, the haploid human genome has 3 x 10 9 bp. Show your calculations. 5. (3 pts) You performed a set of DNA sequencing reactions using the dideoxy method and obtain the gel below. The ddNTP included in each reaction is shown at the top of the gel. Circle the letter for the correct sequence of the template strand of DNA. A. 5'-TGGCTGGCTGAATTC-3' B. 5'-CTTAAGTCGGTCGGT-3' C. 5'-ACCGACCGACTTAAG-3' D. 5'-GAATTCAGCCAGCCA-3' E. NONE OF THE ABOVE
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GENE3200 – Fall 2010 – Bedell Exam 3 – Nov 4 ID no. 810 ______________________________ 2 6. (2 pts) PCR involves 30 – 40 cycles of amplification of a template DNA using oligonucleotide primers and a thermostabile polymerase. There are 3 steps to each cycle and each step is performed at a particular temperature. Which of the following statements is true about PCR? (a) The first step is denaturing of template DNA and occurs at 94 0 C. (b) The second step is DNA synthesis by the polymerase and occurs at 50 - 60 0 C. (c) The third step involves incorporation of ddNTPs. (d) All of the above (e) None of the above 7. (3 pts) Write the sequence for 6 nt primers to use in PCR to amplify the entire DNA sequence shown here. Label each primer "forward" and "reverse". Don't forget the 5' and 3' labels! 5'-TCGATTCGGATTACATCGGCATTACCGATTTAAAGCCCTTACGG-3' 3'-AGCTAAGCCTAATGTAGCCGTAATGGCTAAATTTCGGGAATGCC-5' 8. (2 pts) Which of the following statements IS true of the hierarchical shotgun sequencing strategy to genome sequencing? (a) It requires generation of three genomic libraries cloned into plasmid and BAC vectors. (b)
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Exam3_Fall2010 - GENE3200 Fall 2010 Bedell Exam 3 Nov 4 ID...

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