PHAR, IMMUNO FALL 2010

PHAR, IMMUNO FALL 2010 - DNA AND FORENSICS *DNA profiles...

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DNA AND FORENSICS *DNA profiles what are they? How are they used? Analyzing DNA (two methods)s, kinds of DNA Specific uses then and now (crimes, genealogy, commerce) DNA Profiling: used to ID suspects from hair, blood, semen, other biological materials found at the scne of a violent crime o depends on the fact that no two people (except Identical twins) have the same exactly same DNA sequence. o Exquisisite sensitivity: eye witness ID is only 50% accurate o Application to any body tissue o DNA is stable in comparison to proteins o ID potential suspects o Exonerate accused o ID crime and catastrophe victims o Establish paternity o Disease Susceptibility Two Methods: RFLP analysis and STR analysis o Restriction fragment length polymorphisms : used for analyzing variable lengths of DNA fragments that result from digestion with restriction endonuclease (cutsDNA @ specific sequence). Presence or absence of these sites= fragments of DNA of variable lengths that get separated by size via electrophoresis. Strands are hybridized with radiolabeled DNA probes which bind to complementary sequences. Detect binding via X-Ray film How it works: so DNA is made up of 4 bases (adnenine, thymine, cytosine, and guanine) which are linked together in a strand. The two complementary strands are joined together according to the base pairing rules (AT, GC). =Double stranded DNA. Forensic DNA analysis steps: 1) collect evidence from crime scene. 2) Isolate DNA from evidence sample
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This note was uploaded on 10/05/2011 for the course PHAR IMMUNO taught by Professor - during the Fall '10 term at UConn.

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PHAR, IMMUNO FALL 2010 - DNA AND FORENSICS *DNA profiles...

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